Expression of a humanized viral 2A-mediated lux operon efficiently generates autonomous bioluminescence in human cells.
Expression of autonomous bioluminescence from human cells was previously reported to be impossible, suggesting that all bioluminescent-based mammalian reporter systems must therefore require application of a potentially influential chemical substrate. While this was disproven when the bacterial luci...
Main Authors: | Tingting Xu, Steven Ripp, Gary S Sayler, Dan M Close |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4008522?pdf=render |
Similar Items
-
Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line.
by: Dan M Close, et al.
Published: (2010-08-01) -
Co-Cultured Continuously Bioluminescent Human Cells as Bioreporters for the Detection of Prodrug Therapeutic Impact Pre- and Post-Metabolism
by: Tingting Xu, et al.
Published: (2017-12-01) -
In Vivo Bioluminescent Imaging (BLI): Noninvasive Visualization and Interrogation of Biological Processes in Living Animals
by: Steven Ripp, et al.
Published: (2010-12-01) -
Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells
by: Mark Wienhold, et al.
Published: (2021-11-01) -
The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter
by: Gary Sayler, et al.
Published: (2012-01-01)