pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences

pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences

Increasing demand for large-scale synthesis of in vitro transcribed (IVT) mRNA is being driven by the increasing use of mRNA for transient gene expression in cell engineering and therapeutic applications. An important determinant of IVT mRNA potency is the 3′ polyadenosine (poly(A)) tail, the length...

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Bibliographic Details
Main Authors: Alexandra E Grier, Stephen Burleigh, Jaya Sahni, Courtnee A Clough, Victoire Cardot, Dongwook C Choe, Michelle C Krutein, David J Rawlings, Michael C Jensen, Andrew M Scharenberg, Kyle Jacoby
Format: Article
Language:English
Published: Elsevier 2016-01-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
adenylation
genome engineering
in vitro transcription
mRNA, 3′ untranslated region (UTR)
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117300355

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http://www.sciencedirect.com/science/article/pii/S2162253117300355

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