A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid

A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid

Orotate phosphoribosyltransferase (OPRT) exists as a bifunctional enzyme, uridine 5′-monophosphate synthase, in mammalian cells and plays an important role in pyrimidine biosynthesis. Measuring OPRT activity has been considered important for understanding biological events and development of molecul...

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Bibliographic Details
Main Authors: Takayuki Shibata, Tomohiro Narita, Yutaka Suto, Hasina Yasmin, Tsutomu Kabashima
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Sensors
Subjects:
orotic acid
fluorogenic reaction
orotate phosphoribosyltransferase
enzyme activity
laboratory test
Online Access:https://www.mdpi.com/1424-8220/23/5/2507

Internet

https://www.mdpi.com/1424-8220/23/5/2507

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