The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages
The control of self-renewal and differentiation of neural stem and progenitor cells is a crucial issue in stem cell and cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired neuroblast homeostasis if the limited self-renewing potential of intermediate neural progeni...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-03-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01906 |
| _version_ | 1811181439662686208 |
|---|---|
| author | Chwee Tat Koe Song Li Fabrizio Rossi Jack Jing Lin Wong Yan Wang Zhizhuo Zhang Keng Chen Sherry Shiying Aw Helena E Richardson Paul Robson Wing-Kin Sung Fengwei Yu Cayetano Gonzalez Hongyan Wang |
| author_facet | Chwee Tat Koe Song Li Fabrizio Rossi Jack Jing Lin Wong Yan Wang Zhizhuo Zhang Keng Chen Sherry Shiying Aw Helena E Richardson Paul Robson Wing-Kin Sung Fengwei Yu Cayetano Gonzalez Hongyan Wang |
| author_sort | Chwee Tat Koe |
| collection | DOAJ |
| description | The control of self-renewal and differentiation of neural stem and progenitor cells is a crucial issue in stem cell and cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired neuroblast homeostasis if the limited self-renewing potential of intermediate neural progenitors (INPs) is unrestrained. Here, we demonstrate that Drosophila SWI/SNF chromatin remodeling Brahma (Brm) complex functions cooperatively with another chromatin remodeling factor, Histone deacetylase 3 (HDAC3) to suppress the formation of ectopic type II neuroblasts. We show that multiple components of the Brm complex and HDAC3 physically associate with Earmuff (Erm), a type II-specific transcription factor that prevents dedifferentiation of INPs into neuroblasts. Consistently, the predicted Erm-binding motif is present in most of known binding loci of Brm. Furthermore, brm and hdac3 genetically interact with erm to prevent type II neuroblast overgrowth. Thus, the Brm-HDAC3-Erm repressor complex suppresses dedifferentiation of INPs back into type II neuroblasts. |
| first_indexed | 2024-04-11T09:17:44Z |
| format | Article |
| id | doaj.art-0793e30af78c4f4c8651717beb850fa3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:17:44Z |
| publishDate | 2014-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-0793e30af78c4f4c8651717beb850fa32022-12-22T04:32:16ZengeLife Sciences Publications LtdeLife2050-084X2014-03-01310.7554/eLife.01906The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineagesChwee Tat Koe0Song Li1Fabrizio Rossi2Jack Jing Lin Wong3Yan Wang4Zhizhuo Zhang5Keng Chen6Sherry Shiying Aw7Helena E Richardson8Paul Robson9Wing-Kin Sung10Fengwei Yu11Cayetano Gonzalez12Hongyan Wang13NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore; Neuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, SingaporeNUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore; Neuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, SingaporeCell Division Group, Institute for Research in Biomedicine, Barcelona, SpainNeuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, Singapore; Temasek Life Sciences Laboratory, Singapore, SingaporeNeuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, Singapore; Temasek Life Sciences Laboratory, Singapore, SingaporeDepartment of Computer Science, National University of Singapore, Singapore, SingaporeNUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore; Neuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, SingaporeNUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, SingaporePeter MacCallum Cancer Centre, East Melbourne, Australia; Biochemistry and Molecular Biology Department, University of Melbourne, Parkville, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Australia; Anatomy and Neuroscience Department, University of Melbourne, Parkville, AustraliaGenome Institute of Singapore, Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore, SingaporeGenome Institute of Singapore, Singapore, Singapore; Department of Computer Science, National University of Singapore, Singapore, SingaporeNUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore; Neuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, Singapore; Temasek Life Sciences Laboratory, Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore, SingaporeCell Division Group, Institute for Research in Biomedicine, Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, SpainNUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore; Neuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School Singapore, Singapore, Singapore; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeThe control of self-renewal and differentiation of neural stem and progenitor cells is a crucial issue in stem cell and cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired neuroblast homeostasis if the limited self-renewing potential of intermediate neural progenitors (INPs) is unrestrained. Here, we demonstrate that Drosophila SWI/SNF chromatin remodeling Brahma (Brm) complex functions cooperatively with another chromatin remodeling factor, Histone deacetylase 3 (HDAC3) to suppress the formation of ectopic type II neuroblasts. We show that multiple components of the Brm complex and HDAC3 physically associate with Earmuff (Erm), a type II-specific transcription factor that prevents dedifferentiation of INPs into neuroblasts. Consistently, the predicted Erm-binding motif is present in most of known binding loci of Brm. Furthermore, brm and hdac3 genetically interact with erm to prevent type II neuroblast overgrowth. Thus, the Brm-HDAC3-Erm repressor complex suppresses dedifferentiation of INPs back into type II neuroblasts.https://elifesciences.org/articles/01906neuroblastself-renewaldifferentiationdedifferentiationintermediate neural progenitorDrosophila |
| spellingShingle | Chwee Tat Koe Song Li Fabrizio Rossi Jack Jing Lin Wong Yan Wang Zhizhuo Zhang Keng Chen Sherry Shiying Aw Helena E Richardson Paul Robson Wing-Kin Sung Fengwei Yu Cayetano Gonzalez Hongyan Wang The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages eLife neuroblast self-renewal differentiation dedifferentiation intermediate neural progenitor Drosophila |
| title | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
| title_full | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
| title_fullStr | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
| title_full_unstemmed | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
| title_short | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
| title_sort | brm hdac3 erm repressor complex suppresses dedifferentiation in drosophila type ii neuroblast lineages |
| topic | neuroblast self-renewal differentiation dedifferentiation intermediate neural progenitor Drosophila |
| url | https://elifesciences.org/articles/01906 |
| work_keys_str_mv | AT chweetatkoe thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT songli thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT fabriziorossi thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT jackjinglinwong thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT yanwang thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT zhizhuozhang thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT kengchen thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT sherryshiyingaw thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT helenaerichardson thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT paulrobson thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT wingkinsung thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT fengweiyu thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT cayetanogonzalez thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT hongyanwang thebrmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT chweetatkoe brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT songli brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT fabriziorossi brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT jackjinglinwong brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT yanwang brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT zhizhuozhang brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT kengchen brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT sherryshiyingaw brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT helenaerichardson brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT paulrobson brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT wingkinsung brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT fengweiyu brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT cayetanogonzalez brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages AT hongyanwang brmhdac3ermrepressorcomplexsuppressesdedifferentiationindrosophilatypeiineuroblastlineages |