Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells

Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells

Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approach to facilitate a lean transition from antibody scr...

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Bibliographic Details
Main Authors: David Fiebig, Jan P. Bogen, Stefania C. Carrara, Lukas Deweid, Stefan Zielonka, Julius Grzeschik, Björn Hock, Harald Kolmar
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-05-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
antibody hit discovery
bidirectional promoter
reformatting
golden gate cloning
monoclonal antibodies
yeast surface display
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.794389/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2022.794389/full

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