Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells
Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approach to facilitate a lean transition from antibody scr...
Main Authors: | David Fiebig, Jan P. Bogen, Stefania C. Carrara, Lukas Deweid, Stefan Zielonka, Julius Grzeschik, Björn Hock, Harald Kolmar |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2022-05-01
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Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.794389/full |
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