High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces

High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces

We report here the in vitro selection of DNA aptamers for electric eel acetylcholinesterase (AChE). One selected aptamer sequence (R15/19) has a high affinity towards the enzyme (Kd = 157 ± 42 pM). Characterization of the aptamer showed its binding is not affected by low ionic strength (~20 mM), how...

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Bibliographic Details
Main Authors: Orada Chumphukam, Thao T. Le, Anthony E. G. Cass
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:Molecules
Subjects:
aptamers
acetylcholinesterase
immobilization
Online Access:http://www.mdpi.com/1420-3049/19/4/4986

Internet

http://www.mdpi.com/1420-3049/19/4/4986

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