A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants

A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants

Abstract Background PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality, high molecular weight input DNA. This can be particularly challenging...

Full description

Bibliographic Details
Main Authors: Kanae Nishii, Michael Möller, Robert G. Foster, Laura L. Forrest, Nathan Kelso, Sadie Barber, Caroline Howard, Michelle L. Hart
Format: Article
Language:English
Published: BMC 2023-04-01
Series:Plant Methods
Subjects:
DNA extraction
Genome sequencing
Gesneriaceae
Long-read sequencing
Next-generation sequencing
PacBio HiFi
Online Access:https://doi.org/10.1186/s13007-023-01009-x

Internet

https://doi.org/10.1186/s13007-023-01009-x

Similar Items