Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity

Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity

Real-time PCR was developed to quantify <i>Leishmania infantum</i> kinetoplast DNA and optimized to achieve a sensitivity of 1 parasite/mL. For this purpose, we cloned the conserved kDNA fragment of 120 bp into competent cells and correlated them with serial dilutions of DNA extracted fr...

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Bibliographic Details
Main Authors: Germano Castelli, Federica Bruno, Stefano Reale, Simone Catanzaro, Viviana Valenza, Fabrizio Vitale
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Pathogens
Subjects:
<i>Leishmania infantum</i>
Real-Time PCR
molecular diagnosis
parasite load
Online Access:https://www.mdpi.com/2076-0817/10/7/865

Internet

https://www.mdpi.com/2076-0817/10/7/865

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