Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor
Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5 vvm increased the yield by 16.6% (from 13.81 to 16.1 g/l) upon controlling the temperature at 28 °C. Adjustment of the aeration level at 1...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2013-06-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093113000045 |
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| author | Hewaida F. El-Sedawy Manal M.M. Hussein Tamer Essam Ossama M. El-Tayeb Fatma H.A. Mohammad |
| author_facet | Hewaida F. El-Sedawy Manal M.M. Hussein Tamer Essam Ossama M. El-Tayeb Fatma H.A. Mohammad |
| author_sort | Hewaida F. El-Sedawy |
| collection | DOAJ |
| description | Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5 vvm increased the yield by 16.6% (from 13.81 to 16.1 g/l) upon controlling the temperature at 28 °C. Adjustment of the aeration level at 1.5 vvm for 3 days then controlling the dissolved oxygen (DO) at 30% saturation further increased the yield to 17.8 g/l. The optimum pH was 6.5 for 3 days then 7 thereafter when a production yield of 16.1 g/l was recorded using an aeration rate of 1.5 vvm. Controlling the pH at constant value (6.5 or 7) all through the fermentation process decreased the yield by 5–21%. Controlling the temperature at 30 °C for 3 days then 28 °C thereafter slightly increased the yield by 5% upon using an aeration rate of 1 vvm while it decreased upon using an aeration rate of 1.5 vvm. Integration of the most optimum conditions increased the production yield by 22% from 13.81 to 17.8 g/l. |
| first_indexed | 2024-04-11T02:25:28Z |
| format | Article |
| id | doaj.art-080510df8c94409388b81df69031b209 |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T02:25:28Z |
| publishDate | 2013-06-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-080510df8c94409388b81df69031b2092023-01-02T22:47:03ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312013-06-01511434810.1016/j.bfopcu.2013.02.002Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentorHewaida F. El-Sedawy0Manal M.M. Hussein1Tamer Essam2Ossama M. El-Tayeb3Fatma H.A. Mohammad4Microbial Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, EgyptMicrobial Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, EgyptMicrobial Chemical Engineering and Pilot Plant Department, National Research Center, EgyptOptimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5 vvm increased the yield by 16.6% (from 13.81 to 16.1 g/l) upon controlling the temperature at 28 °C. Adjustment of the aeration level at 1.5 vvm for 3 days then controlling the dissolved oxygen (DO) at 30% saturation further increased the yield to 17.8 g/l. The optimum pH was 6.5 for 3 days then 7 thereafter when a production yield of 16.1 g/l was recorded using an aeration rate of 1.5 vvm. Controlling the pH at constant value (6.5 or 7) all through the fermentation process decreased the yield by 5–21%. Controlling the temperature at 30 °C for 3 days then 28 °C thereafter slightly increased the yield by 5% upon using an aeration rate of 1 vvm while it decreased upon using an aeration rate of 1.5 vvm. Integration of the most optimum conditions increased the production yield by 22% from 13.81 to 17.8 g/l.http://www.sciencedirect.com/science/article/pii/S1110093113000045Amycolatopsis mediterraneiAntibioticsFermentorRifamycin BScaling up |
| spellingShingle | Hewaida F. El-Sedawy Manal M.M. Hussein Tamer Essam Ossama M. El-Tayeb Fatma H.A. Mohammad Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor Bulletin of Faculty of Pharmacy Cairo University Amycolatopsis mediterranei Antibiotics Fermentor Rifamycin B Scaling up |
| title | Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor |
| title_full | Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor |
| title_fullStr | Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor |
| title_full_unstemmed | Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor |
| title_short | Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor |
| title_sort | scaling up for the industrial production of rifamycin b optimization of the process conditions in bench scale fermentor |
| topic | Amycolatopsis mediterranei Antibiotics Fermentor Rifamycin B Scaling up |
| url | http://www.sciencedirect.com/science/article/pii/S1110093113000045 |
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