Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts

Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts

Abstract Background Quantitative RT-PCR is a valuable tool for assessing the gene expression in different human tissues, particularly due to its exceptional sensitivity, accuracy and reliability. However, the choice of adequate control for normalization is a crucial step, greatly affecting the resul...

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Bibliographic Details
Main Authors: Zdenko Červenák, Filip Červenák, Adriana Adamičková, Barbara Šalingová, Andrea Gažová, Ján Kyselovič
Format: Article
Language:English
Published: BMC 2022-04-01
Series:BMC Cardiovascular Disorders
Subjects:
Failing hearts
Left ventricles
qPCR
Reference gene
Normalization
Online Access:https://doi.org/10.1186/s12872-022-02614-9

Internet

https://doi.org/10.1186/s12872-022-02614-9

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