Dissociation of human oral mucosal tissue for single-cell applications

Summary: Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of h...

Full description

Bibliographic Details
Main Authors: Teresa Greenwell-Wild, Drake Winslow Williams, Niki Maria Moutsopoulos
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006146