Dissociation of human oral mucosal tissue for single-cell applications
Summary: Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of h...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006146 |
| _version_ | 1819174603241553920 |
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| author | Teresa Greenwell-Wild Drake Winslow Williams Niki Maria Moutsopoulos |
| author_facet | Teresa Greenwell-Wild Drake Winslow Williams Niki Maria Moutsopoulos |
| author_sort | Teresa Greenwell-Wild |
| collection | DOAJ |
| description | Summary: Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of heterogeneous cell types. This heterogeneous cell suspension can subsequently be used for cytometric analyses or to generate single-cell RNA sequencing libraries.For complete details on the use and execution of this protocol, please refer to Williams et al. (2021). |
| first_indexed | 2024-12-22T20:41:36Z |
| format | Article |
| id | doaj.art-086aae39323a4cc8a5788ad84b7bec5b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T20:41:36Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-086aae39323a4cc8a5788ad84b7bec5b2022-12-21T18:13:19ZengElsevierSTAR Protocols2666-16672021-12-0124100908Dissociation of human oral mucosal tissue for single-cell applicationsTeresa Greenwell-Wild0Drake Winslow Williams1Niki Maria Moutsopoulos2Oral Immunity and Inflammation Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorOral Immunity and Inflammation Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USAOral Immunity and Inflammation Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorSummary: Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of heterogeneous cell types. This heterogeneous cell suspension can subsequently be used for cytometric analyses or to generate single-cell RNA sequencing libraries.For complete details on the use and execution of this protocol, please refer to Williams et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721006146Cell BiologyCell isolationSingle CellHealth SciencesImmunology |
| spellingShingle | Teresa Greenwell-Wild Drake Winslow Williams Niki Maria Moutsopoulos Dissociation of human oral mucosal tissue for single-cell applications STAR Protocols Cell Biology Cell isolation Single Cell Health Sciences Immunology |
| title | Dissociation of human oral mucosal tissue for single-cell applications |
| title_full | Dissociation of human oral mucosal tissue for single-cell applications |
| title_fullStr | Dissociation of human oral mucosal tissue for single-cell applications |
| title_full_unstemmed | Dissociation of human oral mucosal tissue for single-cell applications |
| title_short | Dissociation of human oral mucosal tissue for single-cell applications |
| title_sort | dissociation of human oral mucosal tissue for single cell applications |
| topic | Cell Biology Cell isolation Single Cell Health Sciences Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721006146 |
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