A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors

A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors

An assay for telomerase activity based on asymmetric polymerase chain reaction (A-PCR) on magnetic beads (MBs) and subsequent application of cycling probe technology (CPT) is described. In this assay, the telomerase reaction products are immobilized on MBs, which are then washed to remove PCR inhibi...

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Bibliographic Details
Main Authors: Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto
Format: Article
Language:English
Published: MDPI AG 2013-09-01
Series:Molecules
Subjects:
telomerase
PCR inhibitor
magnetic beads
asymmetric PCR
cycling probe technology
Online Access:http://www.mdpi.com/1420-3049/18/10/11751

Internet

http://www.mdpi.com/1420-3049/18/10/11751

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