A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors
An assay for telomerase activity based on asymmetric polymerase chain reaction (A-PCR) on magnetic beads (MBs) and subsequent application of cycling probe technology (CPT) is described. In this assay, the telomerase reaction products are immobilized on MBs, which are then washed to remove PCR inhibi...
Main Authors: | Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2013-09-01
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Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/18/10/11751 |
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