Biosensor Cell-Fit-HD4D for correlation of single-cell fate and microscale energy deposition in complex ion beams

Biosensor Cell-Fit-HD4D for correlation of single-cell fate and microscale energy deposition in complex ion beams

Summary: We present a protocol for the biosensor Cell-Fit-HD4D. It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging....

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Bibliographic Details
Main Authors: Julian Schlegel, Hans Liew, Katrin Rein, Oleh Dzyubachyk, Jürgen Debus, Amir Abdollahi, Martin Niklas
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Biophysics
Biotechnology and bioengineering
Cancer
Microscopy
Molecular biology
Molecular/Chemical probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006785

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http://www.sciencedirect.com/science/article/pii/S2666166722006785

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