Fluorogenic “click” reaction for labeling and detection of DNA in proliferating cells
A thymidine analog, 5-ethynyl-2′-deoxyuridine (EdU), has been reported as a rapid labeling tool for direct measurement of cells in S-phase. The alkynyl group of EdU is a biologically inert group that undergoes an extremely selective reaction with azido-functionalized groups via Cu(I)-catalyzed alkyn...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2010-07-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000113463 |