Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses
Bioreactor scale-up from the laboratory scale to the industrial scale has always been a pivotal step in bioprocess development. However, the transition of a bioeconomy from innovation to commercialization is often hampered by performance loss in titer, rate and yield. These are often ascribed to tem...
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MDPI AG
2022-01-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/1/45 |
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| author | Qi Yang Wenli Lin Jiawei Xu Nan Guo Jiachen Zhao Gaoya Wang Yongbo Wang Ju Chu Guan Wang |
| author_facet | Qi Yang Wenli Lin Jiawei Xu Nan Guo Jiachen Zhao Gaoya Wang Yongbo Wang Ju Chu Guan Wang |
| author_sort | Qi Yang |
| collection | DOAJ |
| description | Bioreactor scale-up from the laboratory scale to the industrial scale has always been a pivotal step in bioprocess development. However, the transition of a bioeconomy from innovation to commercialization is often hampered by performance loss in titer, rate and yield. These are often ascribed to temporal variations of substrate and dissolved oxygen (for instance) in the environment, experienced by microorganisms at the industrial scale. Oscillations in dissolved oxygen (DO) concentration are not uncommon. Furthermore, these fluctuations can be exacerbated with poor mixing and mass transfer limitations, especially in fermentations with filamentous fungus as the microbial cell factory. In this work, the response of glucose-limited chemostat cultures of an industrial <i>Penicillium chrysogenum</i> strain to different dissolved oxygen levels was assessed under both DO shift-down (60% → 20%, 10% and 5%) and DO ramp-down (60% → 0% in 24 h) conditions. Collectively, the results revealed that the penicillin productivity decreased as the DO level dropped down below 20%, while the byproducts, e.g., 6-oxopiperidine-2-carboxylic acid (OPC) and 6-aminopenicillanic acid (6APA), accumulated. Following DO ramp-down, penicillin productivity under DO shift-up experiments returned to its maximum value in 60 h when the DO was reset to 60%. The result showed that a higher cytosolic redox status, indicated by NADH/NAD+, was observed in the presence of insufficient oxygen supply. Consistent with this, flux balance analysis indicated that the flux through the glyoxylate shunt was increased by a factor of 50 at a DO value of 5% compared to the reference control, favoring the maintenance of redox status. Interestingly, it was observed that, in comparison with the reference control, the penicillin productivity was reduced by 25% at a DO value of 5% under steady state conditions. Only a 14% reduction in penicillin productivity was observed as the DO level was ramped down to 0. Furthermore, intracellular levels of amino acids were less sensitive to DO levels at DO shift-down relative to DO ramp-down conditions; this difference could be caused by different timescales between turnover rates of amino acid pools (tens of seconds to minutes) and DO switches (hours to days at steady state and minutes to hours at ramp-down). In summary, this study showed that changes in oxygen availability can lead to rapid metabolite, flux and productivity responses, and dynamic DO perturbations could provide insight into understanding of metabolic responses in large-scale bioreactors. |
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| issn | 2218-1989 |
| language | English |
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| spelling | doaj.art-08cc3131ca0549a98346679bcf679e172023-11-23T14:40:07ZengMDPI AGMetabolites2218-19892022-01-011214510.3390/metabo12010045Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity ResponsesQi Yang0Wenli Lin1Jiawei Xu2Nan Guo3Jiachen Zhao4Gaoya Wang5Yongbo Wang6Ju Chu7Guan Wang8State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, ChinaBioreactor scale-up from the laboratory scale to the industrial scale has always been a pivotal step in bioprocess development. However, the transition of a bioeconomy from innovation to commercialization is often hampered by performance loss in titer, rate and yield. These are often ascribed to temporal variations of substrate and dissolved oxygen (for instance) in the environment, experienced by microorganisms at the industrial scale. Oscillations in dissolved oxygen (DO) concentration are not uncommon. Furthermore, these fluctuations can be exacerbated with poor mixing and mass transfer limitations, especially in fermentations with filamentous fungus as the microbial cell factory. In this work, the response of glucose-limited chemostat cultures of an industrial <i>Penicillium chrysogenum</i> strain to different dissolved oxygen levels was assessed under both DO shift-down (60% → 20%, 10% and 5%) and DO ramp-down (60% → 0% in 24 h) conditions. Collectively, the results revealed that the penicillin productivity decreased as the DO level dropped down below 20%, while the byproducts, e.g., 6-oxopiperidine-2-carboxylic acid (OPC) and 6-aminopenicillanic acid (6APA), accumulated. Following DO ramp-down, penicillin productivity under DO shift-up experiments returned to its maximum value in 60 h when the DO was reset to 60%. The result showed that a higher cytosolic redox status, indicated by NADH/NAD+, was observed in the presence of insufficient oxygen supply. Consistent with this, flux balance analysis indicated that the flux through the glyoxylate shunt was increased by a factor of 50 at a DO value of 5% compared to the reference control, favoring the maintenance of redox status. Interestingly, it was observed that, in comparison with the reference control, the penicillin productivity was reduced by 25% at a DO value of 5% under steady state conditions. Only a 14% reduction in penicillin productivity was observed as the DO level was ramped down to 0. Furthermore, intracellular levels of amino acids were less sensitive to DO levels at DO shift-down relative to DO ramp-down conditions; this difference could be caused by different timescales between turnover rates of amino acid pools (tens of seconds to minutes) and DO switches (hours to days at steady state and minutes to hours at ramp-down). In summary, this study showed that changes in oxygen availability can lead to rapid metabolite, flux and productivity responses, and dynamic DO perturbations could provide insight into understanding of metabolic responses in large-scale bioreactors.https://www.mdpi.com/2218-1989/12/1/45dissolved oxygenindustrialmetabolomicspenicillin<i>Penicillium chrysogenum</i>scale-down |
| spellingShingle | Qi Yang Wenli Lin Jiawei Xu Nan Guo Jiachen Zhao Gaoya Wang Yongbo Wang Ju Chu Guan Wang Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses Metabolites dissolved oxygen industrial metabolomics penicillin <i>Penicillium chrysogenum</i> scale-down |
| title | Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses |
| title_full | Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses |
| title_fullStr | Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses |
| title_full_unstemmed | Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses |
| title_short | Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of <i>Penicillium chrysogenum</i> Lead to Rapid Metabolite, Flux and Productivity Responses |
| title_sort | changes in oxygen availability during glucose limited chemostat cultivations of i penicillium chrysogenum i lead to rapid metabolite flux and productivity responses |
| topic | dissolved oxygen industrial metabolomics penicillin <i>Penicillium chrysogenum</i> scale-down |
| url | https://www.mdpi.com/2218-1989/12/1/45 |
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