A Method for Radioactive Labelling of Hebeloma cylindrosporum to Study Plant-fungus Interactions

In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum, we supplied 32P to mycelia previously grown in vitro in liquid medium. The culture had four main steps that are 1) growing the mycelium on complete medium with P, 2) transfer the myc...

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Bibliographic Details
Main Authors: Adeline Becquer, Margarita Torres-Aquino, Christine Le Guernevé, Laurie Amenc, Carlos Trives-Segura, Siobhan Staunton, Hervé Quiquampoix, Claude Plassard
Format: Article
Language:English
Published: Bio-protocol LLC 2017-10-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=2576&type=0