Measuring S-Phase Duration from Asynchronous Cells Using Dual EdU-BrdU Pulse-Chase Labeling Flow Cytometry

Measuring S-Phase Duration from Asynchronous Cells Using Dual EdU-BrdU Pulse-Chase Labeling Flow Cytometry

Eukaryotes duplicate their chromosomes during the cell cycle S phase using thousands of initiation sites, tunable fork speed and megabase-long spatio-temporal replication programs. The duration of S phase is fairly constant within a given cell type, but remarkably plastic during development, cell di...

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Bibliographic Details
Main Authors: Marta Bialic, Baraah Al Ahmad Nachar, Maria Koźlak, Vincent Coulon, Etienne Schwob
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Genes
Subjects:
DNA replication
S phase
EdU-BrdU pulse chase
cytometry
cell lines
Online Access:https://www.mdpi.com/2073-4425/13/3/408

Internet

https://www.mdpi.com/2073-4425/13/3/408

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