Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detectio...

Full description

Bibliographic Details
Main Authors: Yingyun Cai, Shuiqing Yu, Ying Fang, Laura Bollinger, Yanhua Li, Michael Lauck, Elena N. Postnikova, Steven Mazur, Reed F. Johnson, Courtney L. Finch, Sheli R. Radoshitzky, Gustavo Palacios, Thomas C. Friedrich, Tony L. Goldberg, David H. O’Connor, Peter B. Jahrling, Jens H. Kuhn
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Viruses
Subjects:
<i>Arteriviridae</i>
cell tropism
infectious clone
<i>Nidovirales</i>
reverse genetics
SHFV
Online Access:https://www.mdpi.com/1999-4915/13/4/632

Internet

https://www.mdpi.com/1999-4915/13/4/632

Similar Items