Electroporation-Based CRISPR/Cas9 Mosaic Mutagenesis of β-Tubulin in the Cultured Oyster

Electroporation-Based CRISPR/Cas9 Mosaic Mutagenesis of β-Tubulin in the Cultured Oyster

Genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is enabling genetics improvement of productive traits in aquaculture. Previous studies have proven CRISPR/Cas9 to be feasible in oyster, one of the most cultured shellfish species. Here, we applied electropo...

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Bibliographic Details
Main Authors: Jiulin Chan, Wei Zhang, Yue Xu, Yu Xue, Linlin Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-05-01
Series:Frontiers in Marine Science
Subjects:
mosaic mutagenesis
CRISPR/Cas9
long deletion
gene editing
gene knockout
aquaculture breeding
Online Access:https://www.frontiersin.org/articles/10.3389/fmars.2022.912409/full

Internet

https://www.frontiersin.org/articles/10.3389/fmars.2022.912409/full

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