Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva

Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva

Abstract Polymerase chain reaction (PCR) has proven to be the gold-standard for SARS-CoV-2 detection in clinical settings. The most common approaches rely on nasopharyngeal specimens obtained from swabs, followed by RNA extraction, reverse transcription and quantitative PCR. Although swab-based PCR...

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Bibliographic Details
Main Authors: Harry H. Jenkins, Ana A. Tellechea Lopez, Francesco Saverio Tarantini, Hannah Tomlin, Danielle Scales, I-Ning Lee, Siyu Wu, Ralph Hyde, Katarzyna Lis-Slimak, Timothy Byaruhanga, Jamie L. Thompson, Sara Pijuan-Galito, Lara Doolan, Kazuyo Kaneko, Penny Gwynne, Caroline Reffin, Emily Park, Jayasree Dey, Jack Hill, Asta Arendt-Tranholm, Amy Stroud, Moira Petrie, Chris Denning, Andrew V. Benest, Claire Seedhouse
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-15616-6

Internet

https://doi.org/10.1038/s41598-022-15616-6

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