Sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-PCR and of HPV by single PCR.

Sexually transmitted diseases (STDs) may impair sperm parameters and functions thereby promoting male infertility. To date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen Thus, we aimed at conceiving and validating a multiplex PCR (M-PCR) assay...

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Main Authors: Fabrícia Gimenes, Fabiana Soares Medina, André Luelsdorf Pimenta de Abreu, Mary Mayumi Taguti Irie, Isis Baroni Esquiçati, Natália Malagutti, Vinícius Rodrigo Bulla Vasconcellos, Michele Garcia Discacciati, Marcelo Gialluisi Bonini, Silvya Stuchi Maria-Engler, Marcia Edilaine Lopes Consolaro
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4055596?pdf=render