scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling

scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling

ABSTRACTCurrent methods for the identification of specific microorganisms based on an in situ metabolism are often hampered by insufficient sensitivity and habitat complexity. Here, we present a novel approach for identifying and sequencing single microbial cells metabolizing a specific organic comp...

Full description

Bibliographic Details
Main Authors: Hao-Yu Lo, Konstantin Wink, Henrike Nitz, Matthias Kästner, Detlev Belder, Jochen A. Müller, Anne-Kristin Kaster
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:mSystems
Subjects:
single-cell sequencing
microautoradiography
microbial metabolism
benzene degradation
FACS
microfluidics
Online Access:https://journals.asm.org/doi/10.1128/msystems.00998-23

Internet

https://journals.asm.org/doi/10.1128/msystems.00998-23

Similar Items