Catalase adsorption onto cibacron blue F3GA-attached chitosan microspheres(活性蓝F3GA固载的壳聚糖微球对过氧化氢酶的吸附)
将反相悬浮交联法制备的壳聚糖微球树脂与三嗪染料活性蓝F3GA (Cibacron Blue F3GA)反应，制得亲和色谱填料，并采用扫描电镜、红外光谱、染料泄漏率测定等对此填料进行鉴定与表征.该填料具有良好的色谱性能，在pH为7.0的磷酸盐缓冲液体系下，对过氧化氢酶的饱和吸附容量为24.6 mg/g，改变pH值及离子强度对结合量有明显影响....
| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2005-11-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2005.32.6.674-677 |