Functional Characterization of Argininosuccinate Lyase Gene Variants by Mini-Gene Splicing Assay

Functional Characterization of Argininosuccinate Lyase Gene Variants by Mini-Gene Splicing Assay

ObjectiveArgininosuccinate lyase (ASL) gene mutations account for argininosuccinic aciduria (ASA). This study aimed to design a minigene construct of ASL gene in order to investigate the impact of variants on splicing.MethodsThe peripheral blood samples were collected from the family members, and ge...

Full description

Bibliographic Details
Main Authors: Yanyun Wang, Yun Sun, Ming Liu, Xiaojuan Zhang, Tao Jiang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Genetics
Subjects:
argininosuccinate lyase (ASL)
exon
intron
argininosuccinic aciduria (ASA)
mini gene
aberrant splicing
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00436/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2019.00436/full

Similar Items