Watson-Crick Base-Pairing Requirements for ssDNA Recognition and Processing in Replication-Initiating HUH Endonucleases

Watson-Crick Base-Pairing Requirements for ssDNA Recognition and Processing in Replication-Initiating HUH Endonucleases

ABSTRACT Replication-initiating HUH endonucleases (Reps) are sequence-specific nucleases that cleave and rejoin single-stranded DNA (ssDNA) during rolling-circle replication. These functions are mediated by covalent linkage of the Rep to its substrate post cleavage. Here, we describe the structures...

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Bibliographic Details
Main Authors: Adam T. Smiley, Kassidy J. Tompkins, Matthew R. Pawlak, August J. Krueger, Robert L. Evans, Ke Shi, Hideki Aihara, Wendy R. Gordon
Format: Article
Language:English
Published: American Society for Microbiology 2023-02-01
Series:mBio
Subjects:
HUH endonuclease
HUH-seq
next-generation sequencing
origin of replication
Rep
Watson-Crick base pair
Online Access:https://journals.asm.org/doi/10.1128/mbio.02587-22

Internet

https://journals.asm.org/doi/10.1128/mbio.02587-22

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