Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri

Contamination of processed beef foods such as abon can be caused by various types of microbes, one of which is Staphylococcus aureus. Staphylococcus aureus can cause various infections, both on the skin, gastrointestinal tract, or endocarditis. The objective of this research was to determine the pre...

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Main Authors: Mastuti Widianingsih, Dian Catur Setyorini
Format: Article
Language:Indonesian
Published: Muhammadiyah University Press 2019-11-01
Series:Bioeksperimen: Jurnal Penelitian Biologi
Subjects:
Online Access:https://journals.ums.ac.id/index.php/bioeksperimen/article/view/9236
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author Mastuti Widianingsih
Dian Catur Setyorini
author_facet Mastuti Widianingsih
Dian Catur Setyorini
author_sort Mastuti Widianingsih
collection DOAJ
description Contamination of processed beef foods such as abon can be caused by various types of microbes, one of which is Staphylococcus aureus. Staphylococcus aureus can cause various infections, both on the skin, gastrointestinal tract, or endocarditis. The objective of this research was to determine the presence of Staphylococcus aureus in beef abon sold in Pahing Market, Kediri. Abon used is non-branded beef abon which is as many as 10 samples obtained by total sampling technique. Samples were tested by observation of colony morphology through Gram staining, mannitol fermentation test, catalase and coagulase test, and acetoin test. The samples were inoculated on Broth NaCl (ink. 24 hour-37°C), then inoculated on MSA (ink. 24 hour-37°C), and VP (ink. 2x24 hours-37°C). Catalase and coagulase tests were carried out by taking colonies on MSA media. The results showed that there were 9 abon samples contaminated with Staphylococcus aureus as indicated by Gram positive staining results, positive (perfect) mannitol fermentation, and positive acetoin, catalase, and coagulase test. The causes of contamination are contaminated abon ingredients, the manufacturing process using less sterile tools, poor handling and processing, processing food with dirty hands, food stored without cover, sick food processors, and dirty markets
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spelling doaj.art-098ca409aa704aabb600d75f0280847c2024-03-27T02:00:24ZindMuhammadiyah University PressBioeksperimen: Jurnal Penelitian Biologi2460-13652527-27992019-11-01529910510.23917/bioeksperimen.v5i2.92364971Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota KediriMastuti Widianingsih0Dian Catur Setyorini1Institut Ilmu Kesehatan Bhakti WiyataInstitut Ilmu Kesehatan Bhakti WiyataContamination of processed beef foods such as abon can be caused by various types of microbes, one of which is Staphylococcus aureus. Staphylococcus aureus can cause various infections, both on the skin, gastrointestinal tract, or endocarditis. The objective of this research was to determine the presence of Staphylococcus aureus in beef abon sold in Pahing Market, Kediri. Abon used is non-branded beef abon which is as many as 10 samples obtained by total sampling technique. Samples were tested by observation of colony morphology through Gram staining, mannitol fermentation test, catalase and coagulase test, and acetoin test. The samples were inoculated on Broth NaCl (ink. 24 hour-37°C), then inoculated on MSA (ink. 24 hour-37°C), and VP (ink. 2x24 hours-37°C). Catalase and coagulase tests were carried out by taking colonies on MSA media. The results showed that there were 9 abon samples contaminated with Staphylococcus aureus as indicated by Gram positive staining results, positive (perfect) mannitol fermentation, and positive acetoin, catalase, and coagulase test. The causes of contamination are contaminated abon ingredients, the manufacturing process using less sterile tools, poor handling and processing, processing food with dirty hands, food stored without cover, sick food processors, and dirty marketshttps://journals.ums.ac.id/index.php/bioeksperimen/article/view/9236abon, contamination, catalase, coagulase, staphylococcus aureus
spellingShingle Mastuti Widianingsih
Dian Catur Setyorini
Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri
Bioeksperimen: Jurnal Penelitian Biologi
abon, contamination, catalase, coagulase, staphylococcus aureus
title Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri
title_full Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri
title_fullStr Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri
title_full_unstemmed Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri
title_short Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri
title_sort identifikasi staphylococcus aureus pada abon sapi di pasar pahing kota kediri
topic abon, contamination, catalase, coagulase, staphylococcus aureus
url https://journals.ums.ac.id/index.php/bioeksperimen/article/view/9236
work_keys_str_mv AT mastutiwidianingsih identifikasistaphylococcusaureuspadaabonsapidipasarpahingkotakediri
AT diancatursetyorini identifikasistaphylococcusaureuspadaabonsapidipasarpahingkotakediri