Development and Single Laboratory Evaluation of a Refined and specific Real-time PCR Detection Method, Using Mitochondrial Primers (Mit1C), for the Detection of Cyclospora cayetanensis in Produce

Development and Single Laboratory Evaluation of a Refined and specific Real-time PCR Detection Method, Using Mitochondrial Primers (Mit1C), for the Detection of Cyclospora cayetanensis in Produce

Regulatory methods for detection of the foodborne protozoan parasite Cyclospora cayetanensis must be specific and sensitive. To that end, we designed and evaluated (in a single laboratory validation) a novel and improved primer/probe combination (Mit1C) for real-time PCR detection of C. cayetanensis...

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Bibliographic Details
Main Authors: Kannan V. Balan, Mark Mammel, David Lipman, Uma Babu, Lisa M. Harrison, Sonia Almeria, Mauricio Durigan, Susan R. Leonard, Hyein Jang, Solomon Gebru, John Grocholl, Socrates Trujillo, Kelli L. Hiett, Steve Musser
Format: Article
Language:English
Published: Elsevier 2023-02-01
Series:Journal of Food Protection
Subjects:
Cyclospora cayetanensis
Foodborne
Leafy greens
Mitochondria
Parasite
Real-time PCR
Online Access:http://www.sciencedirect.com/science/article/pii/S0362028X22119526

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http://www.sciencedirect.com/science/article/pii/S0362028X22119526

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