Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure
Prenatal alcohol exposure disturbs fetal and placental growth and can alter DNA methylation (DNAm). Supplementation with the methyl donor choline can increase fetal and placental growth and restore DNAm, suggesting converging effects on one-carbon metabolism (1CM). We investigated the impact of peri...
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MDPI AG
2022-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/9/1874 |
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| author | Sarah E. Steane Vinod Kumar James S. M. Cuffe Karen M. Moritz Lisa K. Akison |
| author_facet | Sarah E. Steane Vinod Kumar James S. M. Cuffe Karen M. Moritz Lisa K. Akison |
| author_sort | Sarah E. Steane |
| collection | DOAJ |
| description | Prenatal alcohol exposure disturbs fetal and placental growth and can alter DNA methylation (DNAm). Supplementation with the methyl donor choline can increase fetal and placental growth and restore DNAm, suggesting converging effects on one-carbon metabolism (1CM). We investigated the impact of periconceptional ethanol (PCE) exposure and prenatal choline supplementation on 1CM in maternal, placental, and fetal compartments. Female Sprague Dawley rats were given a liquid diet containing 12.5% ethanol (PCE) or 0% ethanol (control) for 4 days before and 4 days after conception. Dams were then placed on chow with different concentrations of choline (1.6 g, 2.6 g, or 7.2 g choline/kg chow). Plasma and tissues were collected in late gestation for the analysis of 1CM components by means of mass spectrometry and real-time PCR. PCE reduced placental components of 1CM, particularly those relating to folate metabolism, resulting in a 3–7.5-fold reduction in the ratio of s-adenosylmethionine:s-adenosylhomocysteine (SAM:SAH) (<i>p</i> < 0.0001). Choline supplementation increased placental 1CM components and the SAM:SAH ratio (3.5–14.5-fold, <i>p</i> < 0.0001). In the maternal and fetal compartments, PCE had little effect, whereas choline increased components of 1CM. This suggests that PCE impairs fetal development via altered placental 1CM, highlighting its role in modulating nutritional inputs to optimize fetal development. |
| first_indexed | 2024-03-10T03:49:24Z |
| format | Article |
| id | doaj.art-0a2779e1b1454b579daa66b957880957 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:49:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-0a2779e1b1454b579daa66b9578809572023-11-23T08:59:45ZengMDPI AGNutrients2072-66432022-04-01149187410.3390/nu14091874Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol ExposureSarah E. Steane0Vinod Kumar1James S. M. Cuffe2Karen M. Moritz3Lisa K. Akison4School of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, AustraliaSchool of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, AustraliaSchool of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, AustraliaSchool of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, AustraliaSchool of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, AustraliaPrenatal alcohol exposure disturbs fetal and placental growth and can alter DNA methylation (DNAm). Supplementation with the methyl donor choline can increase fetal and placental growth and restore DNAm, suggesting converging effects on one-carbon metabolism (1CM). We investigated the impact of periconceptional ethanol (PCE) exposure and prenatal choline supplementation on 1CM in maternal, placental, and fetal compartments. Female Sprague Dawley rats were given a liquid diet containing 12.5% ethanol (PCE) or 0% ethanol (control) for 4 days before and 4 days after conception. Dams were then placed on chow with different concentrations of choline (1.6 g, 2.6 g, or 7.2 g choline/kg chow). Plasma and tissues were collected in late gestation for the analysis of 1CM components by means of mass spectrometry and real-time PCR. PCE reduced placental components of 1CM, particularly those relating to folate metabolism, resulting in a 3–7.5-fold reduction in the ratio of s-adenosylmethionine:s-adenosylhomocysteine (SAM:SAH) (<i>p</i> < 0.0001). Choline supplementation increased placental 1CM components and the SAM:SAH ratio (3.5–14.5-fold, <i>p</i> < 0.0001). In the maternal and fetal compartments, PCE had little effect, whereas choline increased components of 1CM. This suggests that PCE impairs fetal development via altered placental 1CM, highlighting its role in modulating nutritional inputs to optimize fetal development.https://www.mdpi.com/2072-6643/14/9/1874methyl groupone carbon metabolismprenatal alcoholmaternal nutritionplacentamass spectrometry |
| spellingShingle | Sarah E. Steane Vinod Kumar James S. M. Cuffe Karen M. Moritz Lisa K. Akison Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure Nutrients methyl group one carbon metabolism prenatal alcohol maternal nutrition placenta mass spectrometry |
| title | Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure |
| title_full | Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure |
| title_fullStr | Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure |
| title_full_unstemmed | Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure |
| title_short | Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure |
| title_sort | prenatal choline supplementation alters one carbon metabolites in a rat model of periconceptional alcohol exposure |
| topic | methyl group one carbon metabolism prenatal alcohol maternal nutrition placenta mass spectrometry |
| url | https://www.mdpi.com/2072-6643/14/9/1874 |
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