The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome
IntroductionThe antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that re...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227268/full |
| _version_ | 1797652636652011520 |
|---|---|
| author | Javier Marín-Prida Arielis Rodríguez-Ulloa Vladimir Besada Vladimir Besada Alexey Llopiz-Arzuaga Alexey Llopiz-Arzuaga Nathália Vieira Batista Ignacio Hernández-González Nancy Pavón-Fuentes Érica Leandro Marciano Vieira Viviana Falcón-Cama Viviana Falcón-Cama Emilio F. Acosta Gillian Martínez-Donato Majel Cervantes-Llanos Dai Lingfeng Luis J. González Julio Raúl Fernández-Massó Gerardo Guillén-Nieto Gerardo Guillén-Nieto Eduardo Pentón-Arias Eduardo Pentón-Arias Flávio Almeida Amaral Mauro Martins Teixeira Giselle Pentón-Rol Giselle Pentón-Rol |
| author_facet | Javier Marín-Prida Arielis Rodríguez-Ulloa Vladimir Besada Vladimir Besada Alexey Llopiz-Arzuaga Alexey Llopiz-Arzuaga Nathália Vieira Batista Ignacio Hernández-González Nancy Pavón-Fuentes Érica Leandro Marciano Vieira Viviana Falcón-Cama Viviana Falcón-Cama Emilio F. Acosta Gillian Martínez-Donato Majel Cervantes-Llanos Dai Lingfeng Luis J. González Julio Raúl Fernández-Massó Gerardo Guillén-Nieto Gerardo Guillén-Nieto Eduardo Pentón-Arias Eduardo Pentón-Arias Flávio Almeida Amaral Mauro Martins Teixeira Giselle Pentón-Rol Giselle Pentón-Rol |
| author_sort | Javier Marín-Prida |
| collection | DOAJ |
| description | IntroductionThe antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.MethodsAntigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.Results and discussionC-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.ConclusionsThese findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis. |
| first_indexed | 2024-03-11T16:32:50Z |
| format | Article |
| id | doaj.art-0a2d03c8ef5142f4ac5760da86555899 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T16:32:50Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-0a2d03c8ef5142f4ac5760da865558992023-10-23T21:10:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-10-011410.3389/fimmu.2023.12272681227268The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteomeJavier Marín-Prida0Arielis Rodríguez-Ulloa1Vladimir Besada2Vladimir Besada3Alexey Llopiz-Arzuaga4Alexey Llopiz-Arzuaga5Nathália Vieira Batista6Ignacio Hernández-González7Nancy Pavón-Fuentes8Érica Leandro Marciano Vieira9Viviana Falcón-Cama10Viviana Falcón-Cama11Emilio F. Acosta12Gillian Martínez-Donato13Majel Cervantes-Llanos14Dai Lingfeng15Luis J. González16Julio Raúl Fernández-Massó17Gerardo Guillén-Nieto18Gerardo Guillén-Nieto19Eduardo Pentón-Arias20Eduardo Pentón-Arias21Flávio Almeida Amaral22Mauro Martins Teixeira23Giselle Pentón-Rol24Giselle Pentón-Rol25Center for Research and Biological Evaluations, Institute of Pharmacy and Food, University of Havana, Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaChina-Cuba Biotechnology Joint Innovation Center (CCBJIC), Yongzhou Zhong Gu Biotechnology Co. Ltd, Yongzhou, ChinaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDepartment of Cellular Engineering and Biocatalysis , Institute of Biotechnology, National Autonomous University of Mexico (UNAM), Cuernavaca, MexicoLaboratory of Immunopharmacology, Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Non-Clinical Research, Isotopes Center, San José de Las Lajas, Mayabeque, CubaImmunochemical Department, International Center for Neurological Restoration (CIREN), Havana, CubaTranslational Psychoneuroimmunology Group, School of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, BrazilDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDepartments of Physiological or Morphological Sciences, Latin American School of Medicine (ELAM), Havana, Cuba0Department of Characterization, Center for Advanced Studies of Cuba, Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaChina-Cuba Biotechnology Joint Innovation Center (CCBJIC), Yongzhou Zhong Gu Biotechnology Co. Ltd, Yongzhou, ChinaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDepartments of Physiological or Morphological Sciences, Latin American School of Medicine (ELAM), Havana, CubaDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDepartments of Physiological or Morphological Sciences, Latin American School of Medicine (ELAM), Havana, CubaLaboratory of Immunopharmacology, Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilLaboratory of Immunopharmacology, Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDivision of Biomedical Research, Center for Genetic Engineering and Biotechnology, Havana, CubaDepartments of Physiological or Morphological Sciences, Latin American School of Medicine (ELAM), Havana, CubaIntroductionThe antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.MethodsAntigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.Results and discussionC-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.ConclusionsThese findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227268/fullPhycocyanobilinrheumatoid arthritishypernociceptionglutamatergic transmissionproteomeneutrophils |
| spellingShingle | Javier Marín-Prida Arielis Rodríguez-Ulloa Vladimir Besada Vladimir Besada Alexey Llopiz-Arzuaga Alexey Llopiz-Arzuaga Nathália Vieira Batista Ignacio Hernández-González Nancy Pavón-Fuentes Érica Leandro Marciano Vieira Viviana Falcón-Cama Viviana Falcón-Cama Emilio F. Acosta Gillian Martínez-Donato Majel Cervantes-Llanos Dai Lingfeng Luis J. González Julio Raúl Fernández-Massó Gerardo Guillén-Nieto Gerardo Guillén-Nieto Eduardo Pentón-Arias Eduardo Pentón-Arias Flávio Almeida Amaral Mauro Martins Teixeira Giselle Pentón-Rol Giselle Pentón-Rol The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome Frontiers in Immunology Phycocyanobilin rheumatoid arthritis hypernociception glutamatergic transmission proteome neutrophils |
| title | The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_full | The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_fullStr | The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_full_unstemmed | The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_short | The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_sort | effects of phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration inhibition of cytokine production and modulation of the neuronal proteome |
| topic | Phycocyanobilin rheumatoid arthritis hypernociception glutamatergic transmission proteome neutrophils |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227268/full |
| work_keys_str_mv | AT javiermarinprida theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT arielisrodriguezulloa theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vladimirbesada theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vladimirbesada theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT alexeyllopizarzuaga theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT alexeyllopizarzuaga theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT nathaliavieirabatista theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT ignaciohernandezgonzalez theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT nancypavonfuentes theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT ericaleandromarcianovieira theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vivianafalconcama theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vivianafalconcama theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT emiliofacosta theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gillianmartinezdonato theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT majelcervantesllanos theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT dailingfeng theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT luisjgonzalez theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT julioraulfernandezmasso theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gerardoguillennieto theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gerardoguillennieto theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT eduardopentonarias theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT eduardopentonarias theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT flavioalmeidaamaral theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT mauromartinsteixeira theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gisellepentonrol theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gisellepentonrol theeffectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT javiermarinprida effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT arielisrodriguezulloa effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vladimirbesada effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vladimirbesada effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT alexeyllopizarzuaga effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT alexeyllopizarzuaga effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT nathaliavieirabatista effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT ignaciohernandezgonzalez effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT nancypavonfuentes effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT ericaleandromarcianovieira effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vivianafalconcama effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT vivianafalconcama effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT emiliofacosta effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gillianmartinezdonato effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT majelcervantesllanos effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT dailingfeng effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT luisjgonzalez effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT julioraulfernandezmasso effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gerardoguillennieto effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gerardoguillennieto effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT eduardopentonarias effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT eduardopentonarias effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT flavioalmeidaamaral effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT mauromartinsteixeira effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gisellepentonrol effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome AT gisellepentonrol effectsofphycocyanobilinonexperimentalarthritisinvolvethereductioninnociceptionandsynovialneutrophilinfiltrationinhibitionofcytokineproductionandmodulationoftheneuronalproteome |