Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage**
Abstract Flap endonuclease 1 (FEN1) participates in the DNA replication and repair processes, which plays an important role in maintaining genome stability. Determination of FEN1 level is highly required for biochemical studies and clinical diagnosis. Herein, a novel electrochemical approach for the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2023-04-01
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| Series: | ChemElectroChem |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/celc.202300020 |
| _version_ | 1797807354702462976 |
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| author | Dr. Yulin Zhu Dr. Jinwen Zhu Yan Gao Jiayue Shi Prof. Dr. Peng Miao |
| author_facet | Dr. Yulin Zhu Dr. Jinwen Zhu Yan Gao Jiayue Shi Prof. Dr. Peng Miao |
| author_sort | Dr. Yulin Zhu |
| collection | DOAJ |
| description | Abstract Flap endonuclease 1 (FEN1) participates in the DNA replication and repair processes, which plays an important role in maintaining genome stability. Determination of FEN1 level is highly required for biochemical studies and clinical diagnosis. Herein, a novel electrochemical approach for the detection of FEN1 is developed based on clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐associated (Cas) proteins trans‐cleavage. The dumbbell structured DNA substrate, single‐stranded DNA labelled with electrochemical species and three‐dimensional tetrahedral DNA are designed and involved in the reactions. This method displays high sensitivity and good specificity, which can not only monitor FEN1 in various biological samples directly, but also help screen FEN1 inhibitors with potential use for anti‐cancer drug discovery. |
| first_indexed | 2024-03-13T06:21:19Z |
| format | Article |
| id | doaj.art-0a4bcea8a17941c089b7c9669a3c23a9 |
| institution | Directory Open Access Journal |
| issn | 2196-0216 |
| language | English |
| last_indexed | 2024-03-13T06:21:19Z |
| publishDate | 2023-04-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemElectroChem |
| spelling | doaj.art-0a4bcea8a17941c089b7c9669a3c23a92023-06-09T18:22:02ZengWiley-VCHChemElectroChem2196-02162023-04-01108n/an/a10.1002/celc.202300020Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage**Dr. Yulin Zhu0Dr. Jinwen Zhu1Yan Gao2Jiayue Shi3Prof. Dr. Peng Miao4University of Science and Technology of China 230026 Hefei P. R. ChinaUniversity of Science and Technology of China 230026 Hefei P. R. ChinaSuzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences 215163 Suzhou P. R. ChinaSuzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences 215163 Suzhou P. R. ChinaUniversity of Science and Technology of China 230026 Hefei P. R. ChinaAbstract Flap endonuclease 1 (FEN1) participates in the DNA replication and repair processes, which plays an important role in maintaining genome stability. Determination of FEN1 level is highly required for biochemical studies and clinical diagnosis. Herein, a novel electrochemical approach for the detection of FEN1 is developed based on clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐associated (Cas) proteins trans‐cleavage. The dumbbell structured DNA substrate, single‐stranded DNA labelled with electrochemical species and three‐dimensional tetrahedral DNA are designed and involved in the reactions. This method displays high sensitivity and good specificity, which can not only monitor FEN1 in various biological samples directly, but also help screen FEN1 inhibitors with potential use for anti‐cancer drug discovery.https://doi.org/10.1002/celc.202300020enzyme activityCRISPR/Cas12aDNA tetrahedrontriplex-forming oligonucleotidebiosensors |
| spellingShingle | Dr. Yulin Zhu Dr. Jinwen Zhu Yan Gao Jiayue Shi Prof. Dr. Peng Miao Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage** ChemElectroChem enzyme activity CRISPR/Cas12a DNA tetrahedron triplex-forming oligonucleotide biosensors |
| title | Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage** |
| title_full | Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage** |
| title_fullStr | Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage** |
| title_full_unstemmed | Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage** |
| title_short | Electrochemical Determination of Flap Endonuclease 1 Activity Amplified by CRISPR/Cas12a Trans‐Cleavage** |
| title_sort | electrochemical determination of flap endonuclease 1 activity amplified by crispr cas12a trans cleavage |
| topic | enzyme activity CRISPR/Cas12a DNA tetrahedron triplex-forming oligonucleotide biosensors |
| url | https://doi.org/10.1002/celc.202300020 |
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