Purification and Characterization of Nitphym, a Robust Thermostable Nitrilase From Paraburkholderia phymatum

Despite the success of some nitrilases in industrial applications, there is a constant demand to broaden the catalog of these hydrolases, especially robust ones with high operational stability. By using the criteria of thermoresistance to screen a collection of candidate enzymes heterologously expre...

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Bibliographic Details
Main Authors: Thomas Bessonnet, Aline Mariage, Jean-Louis Petit, Virginie Pellouin, Adrien Debard, Anne Zaparucha, Carine Vergne-Vaxelaire, Véronique de Berardinis
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-07-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2021.686362/full