Designed active-site library reveals thousands of functional GFP variants

Designed active-site library reveals thousands of functional GFP variants

Abstract Mutations in a protein active site can lead to dramatic and useful changes in protein activity. The active site, however, is sensitive to mutations due to a high density of molecular interactions, substantially reducing the likelihood of obtaining functional multipoint mutants. We introduce...

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Bibliographic Details
Main Authors: Jonathan Yaacov Weinstein, Carlos Martí-Gómez, Rosalie Lipsh-Sokolik, Shlomo Yakir Hoch, Demian Liebermann, Reinat Nevo, Haim Weissman, Ekaterina Petrovich-Kopitman, David Margulies, Dmitry Ivankov, David M. McCandlish, Sarel J. Fleishman
Format: Article
Language:English
Published: Nature Portfolio 2023-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-38099-z

Internet

https://doi.org/10.1038/s41467-023-38099-z

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