The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide
The present study investigated whether neonatal exposure to the proinflammatory endotoxin lipopolysaccharide (LPS) followed by an antibiotic (ATB)-induced dysbiosis in early adulthood could induce neurodevelopmental disorders-like behavioral changes in adult male rats. Combining these two stressors...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023026245 |
| _version_ | 1797836862484643840 |
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| author | Hana Tejkalová Lea Jakob Simona Kvasnová Jan Klaschka Hana Sechovcová Jakub Mrázek Tomáš Páleníček Kateřina Olša Fliegerová |
| author_facet | Hana Tejkalová Lea Jakob Simona Kvasnová Jan Klaschka Hana Sechovcová Jakub Mrázek Tomáš Páleníček Kateřina Olša Fliegerová |
| author_sort | Hana Tejkalová |
| collection | DOAJ |
| description | The present study investigated whether neonatal exposure to the proinflammatory endotoxin lipopolysaccharide (LPS) followed by an antibiotic (ATB)-induced dysbiosis in early adulthood could induce neurodevelopmental disorders-like behavioral changes in adult male rats. Combining these two stressors resulted in decreased weight gain, but no significant behavioral abnormalities were observed. LPS treatment resulted in adult rats' hypoactivity and induced anxiety-like behavior in the social recognition paradigm, but these behavioral changes were not exacerbated by ATB-induced gut dysbiosis. ATB treatment seriously disrupted the gut bacterial community, but dysbiosis did not affect locomotor activity, social recognition, and acoustic reactivity in adult rats. Fecal bacterial community analyses showed no differences between the LPS challenge exposed/unexposed rats, while the effect of ATB administration was decisive regardless of prior LPS exposure. ATB treatment resulted in significantly decreased bacterial diversity, suppression of Clostridiales and Bacteroidales, and increases in Lactobacillales, Enterobacteriales, and Burkholderiales. The persistent effect of LPS on some aspects of behavior suggests a long-term effect of early toxin exposure that was not observed in ATB-treated animals. However, an anti-inflammatory protective effect of ATB cannot be assumed because of the increased abundance of pro-inflammatory, potentially pathogenic bacteria (Proteus, Suttrella) and the elimination of the bacterial families Ruminococcaceae and Lachnospiraceae, which are generally considered beneficial for gut health. |
| first_indexed | 2024-04-09T15:16:47Z |
| format | Article |
| id | doaj.art-0a89137ff7104c80a3398c9fa26dff67 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T15:16:47Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0a89137ff7104c80a3398c9fa26dff672023-04-29T14:56:40ZengElsevierHeliyon2405-84402023-04-0194e15417The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharideHana Tejkalová0Lea Jakob1Simona Kvasnová2Jan Klaschka3Hana Sechovcová4Jakub Mrázek5Tomáš Páleníček6Kateřina Olša Fliegerová7National Institute of Mental Health; Klecany, Czech RepublicNational Institute of Mental Health; Klecany, Czech Republic; 3rd Faculty of Medicine, Charles University, Czech Republic; Corresponding author. National Institute of Mental Health, Topolova 748, 250 67 Klecany, Czech Republic,Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Czech RepublicInstitute of Computer Science of the Czech Academy of Sciences, Czech RepublicInstitute of Animal Physiology and Genetics of the Czech Academy of Sciences, Czech Republic; Czech University of Life Sciences in Prague, Czech RepublicInstitute of Animal Physiology and Genetics of the Czech Academy of Sciences, Czech RepublicNational Institute of Mental Health; Klecany, Czech Republic; 3rd Faculty of Medicine, Charles University, Czech RepublicInstitute of Animal Physiology and Genetics of the Czech Academy of Sciences, Czech RepublicThe present study investigated whether neonatal exposure to the proinflammatory endotoxin lipopolysaccharide (LPS) followed by an antibiotic (ATB)-induced dysbiosis in early adulthood could induce neurodevelopmental disorders-like behavioral changes in adult male rats. Combining these two stressors resulted in decreased weight gain, but no significant behavioral abnormalities were observed. LPS treatment resulted in adult rats' hypoactivity and induced anxiety-like behavior in the social recognition paradigm, but these behavioral changes were not exacerbated by ATB-induced gut dysbiosis. ATB treatment seriously disrupted the gut bacterial community, but dysbiosis did not affect locomotor activity, social recognition, and acoustic reactivity in adult rats. Fecal bacterial community analyses showed no differences between the LPS challenge exposed/unexposed rats, while the effect of ATB administration was decisive regardless of prior LPS exposure. ATB treatment resulted in significantly decreased bacterial diversity, suppression of Clostridiales and Bacteroidales, and increases in Lactobacillales, Enterobacteriales, and Burkholderiales. The persistent effect of LPS on some aspects of behavior suggests a long-term effect of early toxin exposure that was not observed in ATB-treated animals. However, an anti-inflammatory protective effect of ATB cannot be assumed because of the increased abundance of pro-inflammatory, potentially pathogenic bacteria (Proteus, Suttrella) and the elimination of the bacterial families Ruminococcaceae and Lachnospiraceae, which are generally considered beneficial for gut health.http://www.sciencedirect.com/science/article/pii/S2405844023026245RatsLipopolysaccharideAntibioticsBehaviorMicrobiome |
| spellingShingle | Hana Tejkalová Lea Jakob Simona Kvasnová Jan Klaschka Hana Sechovcová Jakub Mrázek Tomáš Páleníček Kateřina Olša Fliegerová The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide Heliyon Rats Lipopolysaccharide Antibiotics Behavior Microbiome |
| title | The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide |
| title_full | The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide |
| title_fullStr | The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide |
| title_full_unstemmed | The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide |
| title_short | The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide |
| title_sort | influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide |
| topic | Rats Lipopolysaccharide Antibiotics Behavior Microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023026245 |
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