Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model
Abstract Colchicine (COL) is a permeability-glycoprotein (P-gp) substrate drug used for familial Mediterranean fever, acute pericarditis, and the management of acute gout. It has a narrow therapeutic index which implies that a small change in the drug’s absorption profile may lead to either toxicity...
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| Format: | Article |
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Academia Brasileira de Ciências
2022-11-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700902&lng=en&tlng=en |
| _version_ | 1811189176294440960 |
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| author | GERWIN LOUIS T. DELA TORRE ALICE ALMA C. BUNGAY |
| author_facet | GERWIN LOUIS T. DELA TORRE ALICE ALMA C. BUNGAY |
| author_sort | GERWIN LOUIS T. DELA TORRE |
| collection | DOAJ |
| description | Abstract Colchicine (COL) is a permeability-glycoprotein (P-gp) substrate drug used for familial Mediterranean fever, acute pericarditis, and the management of acute gout. It has a narrow therapeutic index which implies that a small change in the drug’s absorption profile may lead to either toxicity or therapeutic failure. Absorption can be altered by modulating the function of P-gp via the concomitant use of drugs, herbal medicines, or food supplements such as probiotics. Here, we investigated the effect of probiotic Lactobacillus acidophilus BIOTECH 1900 on COL’s transepithelial mucosal-to-serosal transport in the jejunum of ICR mice. A high-performance liquid chromatography-photodiode array (HPLC-PDA) method for the assay of COL was developed and validated. The HPLC-PDA method was applied in an ex vivo non-everted gut sac model to measure COL’s cumulative mucosal-to-serosal transport and apparent permeability (Papp). Treatment of L. acidophilus BIOTECH 1900 resulted to a significantly lower COL transport and Papp value compared to the control group. Additionally, the activity of L. acidophilus BIOTECH 1900 was found to be similar to dexamethasone, a known P-gp inducer. We report that L. acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of COL, suggesting possible P-gp induction. Further studies are recommended to substantiate this transporter-based drug-probiotic interaction. |
| first_indexed | 2024-04-11T14:31:35Z |
| format | Article |
| id | doaj.art-0a8ccfbb58cd40ceb0434f826901f954 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T14:31:35Z |
| publishDate | 2022-11-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-0a8ccfbb58cd40ceb0434f826901f9542022-12-22T04:18:34ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902022-11-0194suppl 310.1590/0001-3765202220210766Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac modelGERWIN LOUIS T. DELA TORREhttps://orcid.org/0000-0002-0963-9199ALICE ALMA C. BUNGAYhttps://orcid.org/0000-0002-8670-3350Abstract Colchicine (COL) is a permeability-glycoprotein (P-gp) substrate drug used for familial Mediterranean fever, acute pericarditis, and the management of acute gout. It has a narrow therapeutic index which implies that a small change in the drug’s absorption profile may lead to either toxicity or therapeutic failure. Absorption can be altered by modulating the function of P-gp via the concomitant use of drugs, herbal medicines, or food supplements such as probiotics. Here, we investigated the effect of probiotic Lactobacillus acidophilus BIOTECH 1900 on COL’s transepithelial mucosal-to-serosal transport in the jejunum of ICR mice. A high-performance liquid chromatography-photodiode array (HPLC-PDA) method for the assay of COL was developed and validated. The HPLC-PDA method was applied in an ex vivo non-everted gut sac model to measure COL’s cumulative mucosal-to-serosal transport and apparent permeability (Papp). Treatment of L. acidophilus BIOTECH 1900 resulted to a significantly lower COL transport and Papp value compared to the control group. Additionally, the activity of L. acidophilus BIOTECH 1900 was found to be similar to dexamethasone, a known P-gp inducer. We report that L. acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of COL, suggesting possible P-gp induction. Further studies are recommended to substantiate this transporter-based drug-probiotic interaction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700902&lng=en&tlng=encolchicineHPLC-PDALactobacillus acidophilusnon-everted gut sacP-glycoproteinprobiotics |
| spellingShingle | GERWIN LOUIS T. DELA TORRE ALICE ALMA C. BUNGAY Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model Anais da Academia Brasileira de Ciências colchicine HPLC-PDA Lactobacillus acidophilus non-everted gut sac P-glycoprotein probiotics |
| title | Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model |
| title_full | Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model |
| title_fullStr | Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model |
| title_full_unstemmed | Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model |
| title_short | Lactobacillus acidophilus BIOTECH 1900 decreases the transepithelial mucosal-to-serosal transport of colchicine in an ex vivo non-everted gut sac model |
| title_sort | lactobacillus acidophilus biotech 1900 decreases the transepithelial mucosal to serosal transport of colchicine in an ex vivo non everted gut sac model |
| topic | colchicine HPLC-PDA Lactobacillus acidophilus non-everted gut sac P-glycoprotein probiotics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700902&lng=en&tlng=en |
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