Wheat breeding for the improved bread-making quality using PCR based markers of glutenins
The relation between high-molecular-weight (HMW) glutenin subunits and bread-making quality could enable selection for improved bread-making quality in early stages of breeding process. The composition of HMW glutenin subunits was investigated in F2 and F7 progenies derived from the cross between wi...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2008-09-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200803-0003_wheat-breeding-for-the-improved-bread-making-quality-using-pcr-based-markers-of-glutenins.php |
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| author | Zuzana Kocourková Jana Bradová Zuzana Kohutová Lucie Slámová Pavel Vejl Pavel Horčička |
| author_facet | Zuzana Kocourková Jana Bradová Zuzana Kohutová Lucie Slámová Pavel Vejl Pavel Horčička |
| author_sort | Zuzana Kocourková |
| collection | DOAJ |
| description | The relation between high-molecular-weight (HMW) glutenin subunits and bread-making quality could enable selection for improved bread-making quality in early stages of breeding process. The composition of HMW glutenin subunits was investigated in F2 and F7 progenies derived from the cross between winter wheat varieties Sulamit and Clever. The presence of Glu-A1 (AxNull and Ax1), Glu-B1 (Bx6+By8 and Bx 17+By18) and Glu-D1 alleles (x 5+Dy10 and Dx 2+Dy12) was monitored using a PCR based assay. Segregation of alleles corresponded with the theoretically assumed 1:2:1 Mendelian ratio in F2 generation, however, the values of χ2-test in F7 generation indicated a strong affection of allelic frequencies by the breeding process. Significant variation was also observed in Glu-1 score frequency between F2 and F7 generation. These changes were probably caused by deliberate phenotypic selection for important agronomical traits. SDS and Zeleny sedimentation tests, mixographic parameter breakdown and HMW glutenin composition were analyzed in F7 to reveal the effects of different combinations of HMW glutenin alleles on the bread-making quality characters. The results showed statistically significant differences in the contribution of HMW glutenin alleles. In general, the alleles Ax1, Bx17+By18 and Dx5+Dy 10 can be considered as markers of good baking quality. The data presented in this paper suggest that heterozygous constitution may also have a positive effect on bread-making quality. |
| first_indexed | 2024-04-10T08:30:08Z |
| format | Article |
| id | doaj.art-0abfa0a3f6524c77a5475c7bcbf68ff6 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:30:08Z |
| publishDate | 2008-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-0abfa0a3f6524c77a5475c7bcbf68ff62023-02-23T03:29:20ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252008-09-0144310511310.17221/20/2008-CJGPBcjg-200803-0003Wheat breeding for the improved bread-making quality using PCR based markers of gluteninsZuzana Kocourková0Jana Bradová1Zuzana Kohutová2Lucie Slámová3Pavel Vejl4Pavel Horčička5Plant Breeding Station Stupice, SELGEN, a.s., Stupice, Czech RepublicCrop Research Institute, Prague-Ruzyně, Czech RepublicCzech University of Life Sciences in Prague, Prague, Czech RepublicCzech University of Life Sciences in Prague, Prague, Czech RepublicCzech University of Life Sciences in Prague, Prague, Czech RepublicPlant Breeding Station Stupice, SELGEN, a.s., Stupice, Czech RepublicThe relation between high-molecular-weight (HMW) glutenin subunits and bread-making quality could enable selection for improved bread-making quality in early stages of breeding process. The composition of HMW glutenin subunits was investigated in F2 and F7 progenies derived from the cross between winter wheat varieties Sulamit and Clever. The presence of Glu-A1 (AxNull and Ax1), Glu-B1 (Bx6+By8 and Bx 17+By18) and Glu-D1 alleles (x 5+Dy10 and Dx 2+Dy12) was monitored using a PCR based assay. Segregation of alleles corresponded with the theoretically assumed 1:2:1 Mendelian ratio in F2 generation, however, the values of χ2-test in F7 generation indicated a strong affection of allelic frequencies by the breeding process. Significant variation was also observed in Glu-1 score frequency between F2 and F7 generation. These changes were probably caused by deliberate phenotypic selection for important agronomical traits. SDS and Zeleny sedimentation tests, mixographic parameter breakdown and HMW glutenin composition were analyzed in F7 to reveal the effects of different combinations of HMW glutenin alleles on the bread-making quality characters. The results showed statistically significant differences in the contribution of HMW glutenin alleles. In general, the alleles Ax1, Bx17+By18 and Dx5+Dy 10 can be considered as markers of good baking quality. The data presented in this paper suggest that heterozygous constitution may also have a positive effect on bread-making quality.https://cjgpb.agriculturejournals.cz/artkey/cjg-200803-0003_wheat-breeding-for-the-improved-bread-making-quality-using-pcr-based-markers-of-glutenins.phpbread-making qualitybreedingglutenin subunitsmaswinter wheat |
| spellingShingle | Zuzana Kocourková Jana Bradová Zuzana Kohutová Lucie Slámová Pavel Vejl Pavel Horčička Wheat breeding for the improved bread-making quality using PCR based markers of glutenins Czech Journal of Genetics and Plant Breeding bread-making quality breeding glutenin subunits mas winter wheat |
| title | Wheat breeding for the improved bread-making quality using PCR based markers of glutenins |
| title_full | Wheat breeding for the improved bread-making quality using PCR based markers of glutenins |
| title_fullStr | Wheat breeding for the improved bread-making quality using PCR based markers of glutenins |
| title_full_unstemmed | Wheat breeding for the improved bread-making quality using PCR based markers of glutenins |
| title_short | Wheat breeding for the improved bread-making quality using PCR based markers of glutenins |
| title_sort | wheat breeding for the improved bread making quality using pcr based markers of glutenins |
| topic | bread-making quality breeding glutenin subunits mas winter wheat |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200803-0003_wheat-breeding-for-the-improved-bread-making-quality-using-pcr-based-markers-of-glutenins.php |
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