Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent – the cell extract – remains time-consuming and labor-intensive because of the relatively slow growth ki...

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Príomhchruthaitheoirí: J. Porter Hunt, Emily Long Zhao, Mehran Soltani, Madison Frei, J. Andrew D. Nelson, Bradley C. Bundy
Formáid: Alt
Teanga:English
Foilsithe / Cruthaithe: KeAi Communications Co., Ltd. 2019-12-01
Sraith:Synthetic and Systems Biotechnology
Rochtain ar líne:http://www.sciencedirect.com/science/article/pii/S2405805X19300638