Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent – the cell extract – remains time-consuming and labor-intensive because of the relatively slow growth ki...

Bibliografski detalji
Glavni autori:	J. Porter Hunt, Emily Long Zhao, Mehran Soltani, Madison Frei, J. Andrew D. Nelson, Bradley C. Bundy
Format:	Članak
Jezik:	English
Izdano:	KeAi Communications Co., Ltd. 2019-12-01
Serija:	Synthetic and Systems Biotechnology
Online pristup:	http://www.sciencedirect.com/science/article/pii/S2405805X19300638

Slični predmeti

Highly Efficient Synthesis of Rare Sugars from Glycerol in Endotoxin-Free <i>ClearColi</i> by Fermentation
od: Yahui Gao, i dr.
Izdano: (2023-08-01)

Streamlining the Detection of Human Thyroid Receptor Ligand Interactions with XL1-Blue Cell-Free Protein Synthesis and Beta-Galactosidase Fusion Protein Biosensors
od: J. Porter Hunt, i dr.
Izdano: (2023-09-01)

Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing
od: Prashanta Shrestha, i dr.
Izdano: (2012-09-01)

Erwinia asparaginase (crisantaspase) increases plasma levels of serine and glycine
od: Dominique Bollino, i dr.
Izdano: (2022-12-01)

Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes
od: Helmut Merk, i dr.
Izdano: (2012-09-01)

Bacterial autoinduction: looking outside the cell for new metabolic engineering targets
od: DeLisa Matthew P, i dr.
Izdano: (2002-12-01)

Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli
od: Romel Menacho-Melgar, i dr.
Izdano: (2021-11-01)

A Redox-Based Autoinduction Strategy to Facilitate Expression of 5xCys-Tagged Proteins for Electrobiofabrication
od: Sally Wang, i dr.
Izdano: (2021-06-01)

Mutant analysis of luminescence and autoinduction in a marine bacterium
od: Kuo, Alan J. (Alan James)
Izdano: (2006)

Functionalized Surface Geometries Induce: “Bone: Formation by Autoinduction”
od: Ugo Ripamonti
Izdano: (2018-02-01)

Valproic acid autoinduction: a case-based review
od: Sean Bennett, i dr.
Izdano: (2021-09-01)

The Androgen Hormone-Induced Increase in Androgen Receptor Protein Expression Is Caused by the Autoinduction of the Androgen Receptor Translational Activity
od: Tiziana Siciliano, i dr.
Izdano: (2022-01-01)

Rapid Purification of Endotoxin-Free RTX Toxins
od: Ondrej Stanek, i dr.
Izdano: (2019-06-01)

Plant produced endotoxin binding recombinant proteins effectively remove endotoxins from protein samples
od: Md Rezaul Islam Khan, i dr.
Izdano: (2022-09-01)

Streamlined instrument-free lysis for the detection of Candida auris
od: Mei Jin, i dr.
Izdano: (2023-12-01)

Cell-free chromatin particles released from dying host cells are global instigators of endotoxin sepsis in mice.
od: Indraneel Mittra, i dr.
Izdano: (2020-01-01)

Engineering cell free protein synthesis and synthetic cells
od: Chowdhry, R
Izdano: (2023)

Supramolecular protein assembly in cell-free protein synthesis system
od: Zhixia Li, i dr.
Izdano: (2022-03-01)

Morphological characteristics of free radical processes in endotoxin damage of liver
od: Babanin A.A., i dr.
Izdano: (2009-01-01)

Cell-free protein synthesis : methods and protocols /
od: Spirin, A. S. (Aleksandr Sergeevich), i dr.
Izdano: (2008)

Streamlining the production of proteins for structural biology
od: Owens, R, i dr.
Izdano: (2019)

Endotoxin-free purification for the isolation of Bovine Viral Diarrhoea Virus E2 protein from insoluble inclusion body aggregates
od: Mahony Timothy J, i dr.
Izdano: (2011-07-01)

CAPILLARY MODIFICATION OF THE SINGULARITY AT A FREE-STREAMLINE SEPARATION POINT
od: Cumberbatch, E, i dr.
Izdano: (1979)

A streamlined artificial variable free version of simplex method.
od: Syed Inayatullah, i dr.
Izdano: (2015-01-01)

Pharmacokinetic mechanisms underlying clinical cases of valproic acid autoinduction: A review
od: Narjis Mhaimeed, i dr.
Izdano: (2022-12-01)

A suspected case of autoinduction of voriconazole metabolism in a patient with cerebral aspergillosis
od: Ferguson MJ, i dr.
Izdano: (2017-09-01)

Effective cell-free drug screening protocol for protein-protein interaction
od: Ashkenazi, S, i dr.
Izdano: (2017)

Cell-free Protein Expression of Membrane Proteins Using Nanolipoprotein Particles
od: Federico Katzen
Izdano: (2008-08-01)

Cell-free Protein Expression of Membrane Proteins Using Nanolipoprotein Particles
od: Federico Katzen, i dr.
Izdano: (2008-11-01)

Cell-free Protein Expression of Membrane Proteins Using Nanolipoprotein Particles
od: Federico Katzen, i dr.
Izdano: (2008-10-01)

Cell-Free Protein Synthesis: Chassis toward the Minimal Cell
od: Ke Yue, i dr.
Izdano: (2019-04-01)

Cell-free prediction of protein expression costs for growing cells
od: Olivier Borkowski, i dr.
Izdano: (2018-04-01)

FREE FATTY-ACID METABOLISM DURING CHRONIC ENDOTOXIN INFUSION IN THE RAT
od: Goran, M, i dr.
Izdano: (1986)

Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions
od: Atefeh Sadeghi Shermeh, i dr.
Izdano: (2021-05-01)

Cell-free formation of protease-resistant prion protein
od: Kocisko, David A. (David Allan)
Izdano: (2008)

Silk fibroin as an additive for cell-free protein synthesis
od: Marilyn S. Lee, i dr.
Izdano: (2020-09-01)

Physical stimuli-responsive cell-free protein synthesis
od: Junzhu Yang, i dr.
Izdano: (2020-12-01)

CO2-elevated cell-free protein synthesis
od: Xiaomei Lin, i dr.
Izdano: (2022-09-01)

Cell-Free Expression of a Therapeutic Protein Serratiopeptidase
od: Yaru Meng, i dr.
Izdano: (2023-03-01)

Cell-free protein crystallization for nanocrystal structure determination
od: Satoshi Abe, i dr.
Izdano: (2022-10-01)