Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase
An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent – the cell extract – remains time-consuming and labor-intensive because of the relatively slow growth ki...
| Main Authors: | , , , , , |
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| 格式: | 文件 |
| 语言: | English |
| 出版: |
KeAi Communications Co., Ltd.
2019-12-01
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| 丛编: | Synthetic and Systems Biotechnology |
| 在线阅读: | http://www.sciencedirect.com/science/article/pii/S2405805X19300638 |