Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation
Corylin, a flavonoid isolated from the fruit of <i>Psoralea corylifolia</i>, has an osteogenic effect on osteoblasts in vitro and bone micromass ex vivo. However, the effect and mechanism of corylin in regulating osteoclastogenesis remain unknown. By using murine bone marrow macrophages...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3540 |
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| author | Anna Xiao-Dan Yu Jian Xiao Shi-Zheng Zhao Xiang-Peng Kong Kenneth Kin-Leung Kwan Brody Zhong-Yu Zheng Kevin Qi-Yun Wu Tina Ting-Xia Dong Karl Wah-Keung Tsim |
| author_facet | Anna Xiao-Dan Yu Jian Xiao Shi-Zheng Zhao Xiang-Peng Kong Kenneth Kin-Leung Kwan Brody Zhong-Yu Zheng Kevin Qi-Yun Wu Tina Ting-Xia Dong Karl Wah-Keung Tsim |
| author_sort | Anna Xiao-Dan Yu |
| collection | DOAJ |
| description | Corylin, a flavonoid isolated from the fruit of <i>Psoralea corylifolia</i>, has an osteogenic effect on osteoblasts in vitro and bone micromass ex vivo. However, the effect and mechanism of corylin in regulating osteoclastogenesis remain unknown. By using murine bone marrow macrophages as the osteoclast precursor, corylin was found to inhibit the receptor activator of nuclear factor (NF) κB ligand (RANKL)-induced osteoclast differentiation via down-regulating osteoclastic marker genes. In parallel, F-actin formation and osteoclast migration were diminished in corylin-treated cultured osteoclasts, and subsequently the expressions of osteoclastic proteins were suppressed: the suppression of protein expression was further illustrated by transcriptomic analysis. Furthermore, corylin inhibited the nuclear translocation of p65, giving rise to a restraint in osteoclastic differentiation through the attenuation of transcription factors nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and nuclear factor of activated T cells c1 (NFATc1). There was no obvious change in apoptosis when the RANKL-induce osteoclasts were cultured in the presence of corylin. The finding supports the potential development of corylin as an osteoclast inhibitor against osteoporosis. |
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| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:48:02Z |
| publishDate | 2021-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0ad410fd1e80452eba69d1fa4ef20de02023-11-21T13:18:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227354010.3390/ijms22073540Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast DifferentiationAnna Xiao-Dan Yu0Jian Xiao1Shi-Zheng Zhao2Xiang-Peng Kong3Kenneth Kin-Leung Kwan4Brody Zhong-Yu Zheng5Kevin Qi-Yun Wu6Tina Ting-Xia Dong7Karl Wah-Keung Tsim8Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong 999077, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen 518000, ChinaCorylin, a flavonoid isolated from the fruit of <i>Psoralea corylifolia</i>, has an osteogenic effect on osteoblasts in vitro and bone micromass ex vivo. However, the effect and mechanism of corylin in regulating osteoclastogenesis remain unknown. By using murine bone marrow macrophages as the osteoclast precursor, corylin was found to inhibit the receptor activator of nuclear factor (NF) κB ligand (RANKL)-induced osteoclast differentiation via down-regulating osteoclastic marker genes. In parallel, F-actin formation and osteoclast migration were diminished in corylin-treated cultured osteoclasts, and subsequently the expressions of osteoclastic proteins were suppressed: the suppression of protein expression was further illustrated by transcriptomic analysis. Furthermore, corylin inhibited the nuclear translocation of p65, giving rise to a restraint in osteoclastic differentiation through the attenuation of transcription factors nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and nuclear factor of activated T cells c1 (NFATc1). There was no obvious change in apoptosis when the RANKL-induce osteoclasts were cultured in the presence of corylin. The finding supports the potential development of corylin as an osteoclast inhibitor against osteoporosis.https://www.mdpi.com/1422-0067/22/7/3540corylinosteoclastRANKLtranscriptomicssignaling pathway |
| spellingShingle | Anna Xiao-Dan Yu Jian Xiao Shi-Zheng Zhao Xiang-Peng Kong Kenneth Kin-Leung Kwan Brody Zhong-Yu Zheng Kevin Qi-Yun Wu Tina Ting-Xia Dong Karl Wah-Keung Tsim Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation International Journal of Molecular Sciences corylin osteoclast RANKL transcriptomics signaling pathway |
| title | Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation |
| title_full | Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation |
| title_fullStr | Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation |
| title_full_unstemmed | Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation |
| title_short | Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation |
| title_sort | biological evaluation and transcriptomic analysis of corylin as an inhibitor of osteoclast differentiation |
| topic | corylin osteoclast RANKL transcriptomics signaling pathway |
| url | https://www.mdpi.com/1422-0067/22/7/3540 |
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