Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental out...

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Bibliographic Details
Main Authors: Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink, Barbara Rothen-Rutishauser
Format: Article
Language:English
Published: BMC 2022-10-01
Series:Journal of Nanobiotechnology
Subjects:
Nanoparticles
Lysosomes
LysoTracker probes
Colocalization
Macrophages
Pitfalls
Online Access:https://doi.org/10.1186/s12951-022-01670-9

Internet

https://doi.org/10.1186/s12951-022-01670-9

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