Assessing the Orthogonality of Phage-Encoded RNA Polymerases for Tailored Synthetic Biology Applications in <i>Pseudomonas</i> Species

Assessing the Orthogonality of Phage-Encoded RNA Polymerases for Tailored Synthetic Biology Applications in <i>Pseudomonas</i> Species

The phage T7 RNA polymerase (RNAP) and lysozyme form the basis of the widely used pET expression system for recombinant expression in the biotechnology field and as a tool in microbial synthetic biology. Attempts to transfer this genetic circuitry from <i>Escherichia coli</i> to non-mode...

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Bibliographic Details
Main Authors: Eveline-Marie Lammens, Nathalie Feyaerts, Alison Kerremans, Maarten Boon, Rob Lavigne
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Pseudomonas</i>
T7-like phages
RNA polymerase
lysozyme
orthogonality
Online Access:https://www.mdpi.com/1422-0067/24/8/7175

Internet

https://www.mdpi.com/1422-0067/24/8/7175

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