Identification of an N-terminal tag (580N) that improves the biosynthesis of fluorescent proteins in Francisella tularensis and other Gram-negative bacteria

Identification of an N-terminal tag (580N) that improves the biosynthesis of fluorescent proteins in Francisella tularensis and other Gram-negative bacteria

Utilization of fluorescent proteins is widespread for the study of microbial pathogenesis and host-pathogen interactions. Here, we discovered that linkage of the 36 N-terminal amino acids of FTL_0580 (a hypothetical protein of Francisella tularensis) to fluorescent proteins increases the fluorescenc...

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Bibliographic Details
Main Authors: Kristen Haggerty, Stuart Cantlay, Emily Young, Mariah K. Cashbaugh, Elio F. Delatore III, Rori Schreiber, Hayden Hess, Daniel R. Komlosi, Sarah Butler, Dalton Bolon, Theresa Evangelista, Takoda Hager, Claire Kelly, Katherine Phillips, Jada Voellinger, Robert M.Q. Shanks, Joseph Horzempa
Format: Article
Language:English
Published: Elsevier 2024-04-01
Series:Molecular and Cellular Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S0890850824000082

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http://www.sciencedirect.com/science/article/pii/S0890850824000082

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