Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Abstract Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food. These methods rely on the detection of gene sequences to indicate the presence of viable bacteria. This...

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Bibliographic Details
Main Authors: Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber
Format: Article
Language:English
Published: BMC 2022-09-01
Series:BMC Microbiology
Subjects:
16S rRNA amplicon sequencing
Bacterial culture
Culture independent detection
Biotyper
DNA extraction
Metagenomics
Online Access:https://doi.org/10.1186/s12866-022-02650-7

Internet

https://doi.org/10.1186/s12866-022-02650-7

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