Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients
Focal cortical dysplasia (FCD) is one of the most common causes of drug-resistant epilepsy. As several studies have revealed, the abnormal functioning of the perisomatic inhibitory system may play a role in the onset of seizures. Therefore, we wanted to investigate whether changes of perisomatic inh...
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2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Cecília Szekeres-Paraczky Péter Szocsics Loránd Erőss Dániel Fabó László Mód Zsófia Maglóczky |
| author_facet | Cecília Szekeres-Paraczky Péter Szocsics Loránd Erőss Dániel Fabó László Mód Zsófia Maglóczky |
| author_sort | Cecília Szekeres-Paraczky |
| collection | DOAJ |
| description | Focal cortical dysplasia (FCD) is one of the most common causes of drug-resistant epilepsy. As several studies have revealed, the abnormal functioning of the perisomatic inhibitory system may play a role in the onset of seizures. Therefore, we wanted to investigate whether changes of perisomatic inhibitory inputs are present in FCD. Thus, the input properties of abnormal giant- and control-like principal cells were examined in FCD type IIB patients. Surgical samples were compared to controls from the same cortical regions with short postmortem intervals. For the study, six subjects were selected/each group. The perisomatic inhibitory terminals were quantified in parvalbumin and neuronal nuclei double immunostained sections using a confocal fluorescent microscope. The perisomatic input of giant neurons was extremely abundant, whereas control-like cells of the same samples had sparse inputs. A comparison of pooled data shows that the number of parvalbumin-immunopositive perisomatic terminals contacting principal cells was significantly larger in epileptic cases. The analysis showed some heterogeneity among epileptic samples. However, five out of six cases had significantly increased perisomatic input. Parameters of the control cells were homogenous. The reorganization of the perisomatic inhibitory system may increase the probability of seizure activity and might be a general mechanism of abnormal network activity. |
| first_indexed | 2024-03-10T04:05:54Z |
| format | Article |
| id | doaj.art-0bb0ed1c48d845eeba008cdefa831999 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:05:54Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0bb0ed1c48d845eeba008cdefa8319992023-11-23T08:21:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239474610.3390/ijms23094746Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic PatientsCecília Szekeres-Paraczky0Péter Szocsics1Loránd Erőss2Dániel Fabó3László Mód4Zsófia Maglóczky5Human Brain Research Laboratory, Institute of Experimental Medicine, ELKH, 1083 Budapest, HungaryHuman Brain Research Laboratory, Institute of Experimental Medicine, ELKH, 1083 Budapest, HungaryDepartment of Functional Neurosurgery and Center of Neuromodulation, National Institute of Mental Health Neurology and Neurosurgery, 1145 Budapest, HungaryDepartment of Functional Neurosurgery and Center of Neuromodulation, National Institute of Mental Health Neurology and Neurosurgery, 1145 Budapest, HungaryDepartment of Pathology, St. Borbála Hospital, 2800 Tatabánya, HungaryHuman Brain Research Laboratory, Institute of Experimental Medicine, ELKH, 1083 Budapest, HungaryFocal cortical dysplasia (FCD) is one of the most common causes of drug-resistant epilepsy. As several studies have revealed, the abnormal functioning of the perisomatic inhibitory system may play a role in the onset of seizures. Therefore, we wanted to investigate whether changes of perisomatic inhibitory inputs are present in FCD. Thus, the input properties of abnormal giant- and control-like principal cells were examined in FCD type IIB patients. Surgical samples were compared to controls from the same cortical regions with short postmortem intervals. For the study, six subjects were selected/each group. The perisomatic inhibitory terminals were quantified in parvalbumin and neuronal nuclei double immunostained sections using a confocal fluorescent microscope. The perisomatic input of giant neurons was extremely abundant, whereas control-like cells of the same samples had sparse inputs. A comparison of pooled data shows that the number of parvalbumin-immunopositive perisomatic terminals contacting principal cells was significantly larger in epileptic cases. The analysis showed some heterogeneity among epileptic samples. However, five out of six cases had significantly increased perisomatic input. Parameters of the control cells were homogenous. The reorganization of the perisomatic inhibitory system may increase the probability of seizure activity and might be a general mechanism of abnormal network activity.https://www.mdpi.com/1422-0067/23/9/4746perisomatic inhibitiondysmorphic neuronsfocal epilepsyhuman cortexparvalbuminFCD |
| spellingShingle | Cecília Szekeres-Paraczky Péter Szocsics Loránd Erőss Dániel Fabó László Mód Zsófia Maglóczky Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients International Journal of Molecular Sciences perisomatic inhibition dysmorphic neurons focal epilepsy human cortex parvalbumin FCD |
| title | Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients |
| title_full | Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients |
| title_fullStr | Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients |
| title_full_unstemmed | Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients |
| title_short | Reorganization of Parvalbumin Immunopositive Perisomatic Innervation of Principal Cells in Focal Cortical Dysplasia Type IIB in Human Epileptic Patients |
| title_sort | reorganization of parvalbumin immunopositive perisomatic innervation of principal cells in focal cortical dysplasia type iib in human epileptic patients |
| topic | perisomatic inhibition dysmorphic neurons focal epilepsy human cortex parvalbumin FCD |
| url | https://www.mdpi.com/1422-0067/23/9/4746 |
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