Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria
<i>Sanguisorba minor</i> is a medicinal vegetable used in seasoning desserts, juices, and beverages. An evaluation of the total flavonoid, phenolic, tannin and anthocyanin contents indicated that these classes of compounds are distributed variably in the different fractions. In summary,...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/24/4134 |
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| author | Chahrazed Haouam Sameh Boudiba Alfred Ngenge Tamfu Selcuk Kucukaydin Karima Hanini Haouaouchi Fatma Zohra Soraya Hioun Andreea Dediu Botezatu Özgür Ceylan Louiza Boudiba Mehmet Emin Duru Rodica Mihaela Dinica |
| author_facet | Chahrazed Haouam Sameh Boudiba Alfred Ngenge Tamfu Selcuk Kucukaydin Karima Hanini Haouaouchi Fatma Zohra Soraya Hioun Andreea Dediu Botezatu Özgür Ceylan Louiza Boudiba Mehmet Emin Duru Rodica Mihaela Dinica |
| author_sort | Chahrazed Haouam |
| collection | DOAJ |
| description | <i>Sanguisorba minor</i> is a medicinal vegetable used in seasoning desserts, juices, and beverages. An evaluation of the total flavonoid, phenolic, tannin and anthocyanin contents indicated that these classes of compounds are distributed variably in the different fractions. In summary, the HPLC-DAD analyses enabled the identification and quantification of thirteen phenolic compounds in an ethyl acetate extract (EAE), nine in a dichloromethane extract (DCME), seven in an aqueous extract (AQE) and four in a butanol extract (BE). Rutin was the most abundant phenolic compound in the BE (278.4 ± 1.20 µg/g) and AQE (32.87 ± 0.23 µg/g) fractions, while apigenin was the most abundant in the DCME (84.75 ± 0.60 µg/g) and EAE (156.8 ± 0.95 µg/g) fractions. The presence of phenolic compounds in the fractions conferred good antioxidant capacity, especially the EAE and DCME fractions, which both exhibited higher antioxidant effects than BHA and α-tocopherol in DPPH<sup>•</sup> and CUPRAC assays. Additionally, in the ABTS<sup>•+</sup> assay, EAE (IC<sub>50</sub> = 9.27 ± 0.33 µg/mL) was more active than α-tocopherol (IC<sub>50</sub> = 35.50 ± 0.55 µg/mL), and BHA (IC<sub>50</sub> = 12.70 ± 0.10 µg/mL). At 200 µg/mL, the fractions inhibited acetylcholinesterase and butyrylcholinesterase as well as α-amylase and α-glucosidase, indicating that they can slow neurodegeneration and hyperglycemia. Minimal inhibitory concentration (MIC) values ranged from 0.312 mg/mL to 1.25 mg/mL, and fractions showed good biofilm inhibition against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The extracts exhibited good violacein inhibition in <i>Chromobacterium violaceum</i> CV12472 and <i>Chromobacterium violaceum</i> CV026, despite the supply of external acyl-homoserine lactone to CV026. The antioxidant, quorum-sensing, antibiofilm and enzyme inhibition attributes indicate the potential for the application of <i>S. minor</i> as a food preservative. |
| first_indexed | 2024-03-08T18:34:18Z |
| format | Article |
| id | doaj.art-0be46e4202e34f96a414562f43849c16 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T18:34:18Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-0be46e4202e34f96a414562f43849c162023-12-29T15:47:28ZengMDPI AGPlants2223-77472023-12-011224413410.3390/plants12244134Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from AlgeriaChahrazed Haouam0Sameh Boudiba1Alfred Ngenge Tamfu2Selcuk Kucukaydin3Karima Hanini4Haouaouchi Fatma Zohra5Soraya Hioun6Andreea Dediu Botezatu7Özgür Ceylan8Louiza Boudiba9Mehmet Emin Duru10Rodica Mihaela Dinica11Laboratory of Applied Chemistry and Renewable Energies (LACRE), Echahid Cheikh Larbi Tebessi University, Constantine Road, Tebessa 12002, AlgeriaLaboratory of Applied Chemistry and Renewable Energies (LACRE), Echahid Cheikh Larbi Tebessi University, Constantine Road, Tebessa 12002, AlgeriaDepartment of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere 454, CameroonDepartment of Chemistry, Faculty of Science, Mugla Sitki Kocman University, Mugla 48000, TurkeyLaboratory of Applied Chemistry and Renewable Energies (LACRE), Echahid Cheikh Larbi Tebessi University, Constantine Road, Tebessa 12002, AlgeriaLaboratory of Applied Chemistry and Renewable Energies (LACRE), Echahid Cheikh Larbi Tebessi University, Constantine Road, Tebessa 12002, AlgeriaDepartment of Natural and Life Sciences FSESNV, Echahid Cheikh Larbi Tebessi University, Constantine Road, Tebessa 12002, AlgeriaDepartment of Chemistry, Physics and Environment, Faculty of Sciences and Environment, Dunarea de Jos University, 47 Domneasca Str., 800008 Galati, RomaniaFood Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla 48147, TurkeyLaboratory of Applied Chemistry and Renewable Energies (LACRE), Echahid Cheikh Larbi Tebessi University, Constantine Road, Tebessa 12002, AlgeriaDepartment of Chemistry, Faculty of Science, Mugla Sitki Kocman University, Mugla 48000, TurkeyDepartment of Chemistry, Physics and Environment, Faculty of Sciences and Environment, Dunarea de Jos University, 47 Domneasca Str., 800008 Galati, Romania<i>Sanguisorba minor</i> is a medicinal vegetable used in seasoning desserts, juices, and beverages. An evaluation of the total flavonoid, phenolic, tannin and anthocyanin contents indicated that these classes of compounds are distributed variably in the different fractions. In summary, the HPLC-DAD analyses enabled the identification and quantification of thirteen phenolic compounds in an ethyl acetate extract (EAE), nine in a dichloromethane extract (DCME), seven in an aqueous extract (AQE) and four in a butanol extract (BE). Rutin was the most abundant phenolic compound in the BE (278.4 ± 1.20 µg/g) and AQE (32.87 ± 0.23 µg/g) fractions, while apigenin was the most abundant in the DCME (84.75 ± 0.60 µg/g) and EAE (156.8 ± 0.95 µg/g) fractions. The presence of phenolic compounds in the fractions conferred good antioxidant capacity, especially the EAE and DCME fractions, which both exhibited higher antioxidant effects than BHA and α-tocopherol in DPPH<sup>•</sup> and CUPRAC assays. Additionally, in the ABTS<sup>•+</sup> assay, EAE (IC<sub>50</sub> = 9.27 ± 0.33 µg/mL) was more active than α-tocopherol (IC<sub>50</sub> = 35.50 ± 0.55 µg/mL), and BHA (IC<sub>50</sub> = 12.70 ± 0.10 µg/mL). At 200 µg/mL, the fractions inhibited acetylcholinesterase and butyrylcholinesterase as well as α-amylase and α-glucosidase, indicating that they can slow neurodegeneration and hyperglycemia. Minimal inhibitory concentration (MIC) values ranged from 0.312 mg/mL to 1.25 mg/mL, and fractions showed good biofilm inhibition against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The extracts exhibited good violacein inhibition in <i>Chromobacterium violaceum</i> CV12472 and <i>Chromobacterium violaceum</i> CV026, despite the supply of external acyl-homoserine lactone to CV026. The antioxidant, quorum-sensing, antibiofilm and enzyme inhibition attributes indicate the potential for the application of <i>S. minor</i> as a food preservative.https://www.mdpi.com/2223-7747/12/24/4134<i>Sanguisorba minor</i>chemical compositionantioxidant activityantibiofilm propertiesanticholinesterase activityantidiabetic potential |
| spellingShingle | Chahrazed Haouam Sameh Boudiba Alfred Ngenge Tamfu Selcuk Kucukaydin Karima Hanini Haouaouchi Fatma Zohra Soraya Hioun Andreea Dediu Botezatu Özgür Ceylan Louiza Boudiba Mehmet Emin Duru Rodica Mihaela Dinica Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria Plants <i>Sanguisorba minor</i> chemical composition antioxidant activity antibiofilm properties anticholinesterase activity antidiabetic potential |
| title | Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria |
| title_full | Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria |
| title_fullStr | Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria |
| title_full_unstemmed | Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria |
| title_short | Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (<i>Sanguisorba minor</i> L.) Harvested from Algeria |
| title_sort | assessment of chemical composition and in vitro antioxidant antidiabetic anticholinesterase and microbial virulence quenching effects of salad burnet i sanguisorba minor i l harvested from algeria |
| topic | <i>Sanguisorba minor</i> chemical composition antioxidant activity antibiofilm properties anticholinesterase activity antidiabetic potential |
| url | https://www.mdpi.com/2223-7747/12/24/4134 |
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