Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle.

Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle.

Here we report the monitoring of the digestive tract colonization of Rhodnius prolixus by Trypanosoma cruzi using an accurate determination of the parasite load by qPCR coupled with fluorescence and bioluminescence imaging (BLI). These complementary methods revealed critical steps necessary for the...

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Bibliographic Details
Main Authors: Felipe de Almeida Dias, Barbara Guerra, Larissa Rezende Vieira, Hugo Diego Perdomo, Ana Caroline Paiva Gandara, Raquel Juliana Vionette do Amaral, Renata Estebanez Vollú, Suzete Araujo Oliveira Gomes, Flavio Alves Lara, Marcos Henrique Ferreira Sorgine, Emiliano Medei, Pedro Lagerblad de Oliveira, Didier Salmon
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC4619730?pdf=render

Internet

http://europepmc.org/articles/PMC4619730?pdf=render

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