Targeted repression of topA by CRISPRi reveals a critical function for balanced DNA topoisomerase I activity in the Chlamydia trachomatis developmental cycle

Targeted repression of topA by CRISPRi reveals a critical function for balanced DNA topoisomerase I activity in the Chlamydia trachomatis developmental cycle

ABSTRACTChlamydia trachomatis is an obligate intracellular bacterium that is responsible for the most prevalent bacterial sexually transmitted infection. Changes in DNA topology in this pathogen have been linked to its pathogenicity-associated developmental cycle. Here, evidence is provided that the...

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Bibliographic Details
Main Authors: Li Shen, Leiqiong Gao, Abigail R. Swoboda, Scot P. Ouellette
Format: Article
Language:English
Published: American Society for Microbiology 2024-02-01
Series:mBio
Subjects:
Chlamydia trachomatis
DNA topoisomerase
topA
CRISPRi
dCas12
bacterial developmental cycle
Online Access:https://journals.asm.org/doi/10.1128/mbio.02584-23

Internet

https://journals.asm.org/doi/10.1128/mbio.02584-23

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